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Protocol for base resolution mapping of ac4C using RedaC:T-seq

N4-acetylcytidine (ac4C) is an mRNA modification catalyzed by the enzyme N-acetyltransferase 10 (NAT10), with position-dependent effects on mRNA translation. This protocol details a procedure to map ac4C at base resolution using NaBH(4)-induced reduction of ac4C and conversion to thymidine followed...

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Detalles Bibliográficos
Autores principales: Sturgill, David, Arango, Daniel, Oberdoerffer, Shalini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676198/
https://www.ncbi.nlm.nih.gov/pubmed/36595942
http://dx.doi.org/10.1016/j.xpro.2022.101858