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Protocol for base resolution mapping of ac4C using RedaC:T-seq
N4-acetylcytidine (ac4C) is an mRNA modification catalyzed by the enzyme N-acetyltransferase 10 (NAT10), with position-dependent effects on mRNA translation. This protocol details a procedure to map ac4C at base resolution using NaBH(4)-induced reduction of ac4C and conversion to thymidine followed...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676198/ https://www.ncbi.nlm.nih.gov/pubmed/36595942 http://dx.doi.org/10.1016/j.xpro.2022.101858 |