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Non-invasive chimeric HaloTag labeling to study clustering and diffusion of membrane proteins
As live imaging plays an increasingly critical role in cell biology research, the desire to label and track individual protein molecules in vivo has been growing. To address this, in this protocol we describe steps for sparse labeling using two different HaloTag ligand dyes in C. elegans. This label...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676207/ https://www.ncbi.nlm.nih.gov/pubmed/36595905 http://dx.doi.org/10.1016/j.xpro.2022.101857 |