Protocol for targeted modification of the rice genome using base editing

Base editing is a precision genome-editing approach that is widely utilized to generate single-nucleotide variants (SNVs) in genomes. Here, we present a protocol to perform targeted adenine (A)-to-guanine (G) substitution in rice using adenine base editor (ABE). We detail the design of sgRNA, CRISPR...

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Detalles Bibliográficos
Autores principales: Wang, Meixia, Yan, Fang, Zhou, Huanbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676627/
https://www.ncbi.nlm.nih.gov/pubmed/36595935
http://dx.doi.org/10.1016/j.xpro.2022.101865

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676627/
https://www.ncbi.nlm.nih.gov/pubmed/36595935
http://dx.doi.org/10.1016/j.xpro.2022.101865

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