Protocol for targeted modification of the rice genome using base editing

Base editing is a precision genome-editing approach that is widely utilized to generate single-nucleotide variants (SNVs) in genomes. Here, we present a protocol to perform targeted adenine (A)-to-guanine (G) substitution in rice using adenine base editor (ABE). We detail the design of sgRNA, CRISPR...

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Detalles Bibliográficos
Autores principales: Wang, Meixia, Yan, Fang, Zhou, Huanbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676627/
https://www.ncbi.nlm.nih.gov/pubmed/36595935
http://dx.doi.org/10.1016/j.xpro.2022.101865
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author Wang, Meixia
Yan, Fang
Zhou, Huanbin
author_facet Wang, Meixia
Yan, Fang
Zhou, Huanbin
author_sort Wang, Meixia
collection PubMed
description Base editing is a precision genome-editing approach that is widely utilized to generate single-nucleotide variants (SNVs) in genomes. Here, we present a protocol to perform targeted adenine (A)-to-guanine (G) substitution in rice using adenine base editor (ABE). We detail the design of sgRNA, CRISPR plasmid construction, rapid genetic transformation of rice, and genotyping of editing events. This protocol can be applied to cytosine base editing in rice as well. For complete details on the use and execution of this protocol, please refer to Yan et al. (2021).(1)
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spelling pubmed-96766272022-11-22 Protocol for targeted modification of the rice genome using base editing Wang, Meixia Yan, Fang Zhou, Huanbin STAR Protoc Protocol Base editing is a precision genome-editing approach that is widely utilized to generate single-nucleotide variants (SNVs) in genomes. Here, we present a protocol to perform targeted adenine (A)-to-guanine (G) substitution in rice using adenine base editor (ABE). We detail the design of sgRNA, CRISPR plasmid construction, rapid genetic transformation of rice, and genotyping of editing events. This protocol can be applied to cytosine base editing in rice as well. For complete details on the use and execution of this protocol, please refer to Yan et al. (2021).(1) Elsevier 2022-11-19 /pmc/articles/PMC9676627/ /pubmed/36595935 http://dx.doi.org/10.1016/j.xpro.2022.101865 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wang, Meixia
Yan, Fang
Zhou, Huanbin
Protocol for targeted modification of the rice genome using base editing
title Protocol for targeted modification of the rice genome using base editing
title_full Protocol for targeted modification of the rice genome using base editing
title_fullStr Protocol for targeted modification of the rice genome using base editing
title_full_unstemmed Protocol for targeted modification of the rice genome using base editing
title_short Protocol for targeted modification of the rice genome using base editing
title_sort protocol for targeted modification of the rice genome using base editing
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676627/
https://www.ncbi.nlm.nih.gov/pubmed/36595935
http://dx.doi.org/10.1016/j.xpro.2022.101865
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AT zhouhuanbin protocolfortargetedmodificationofthericegenomeusingbaseediting

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