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Lrig1 regulates the balance between proliferation and quiescence in glioblastoma stem cells

Patients with glioblastoma (GBM) face a dismal prognosis. GBMs are driven by glioblastoma stem cells (GSCs) that display a neural stem cell (NSC)-like phenotype. These glioblastoma stem cells are often in a quiescent state that evades current therapies, namely debulking surgery and chemo/radiotherap...

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Autores principales: Ferguson, Kirsty M., Blin, Carla, Alfazema, Neza, Gangoso, Ester, Pollard, Steven M., Marques-Torrejon, Maria Angeles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677454/
https://www.ncbi.nlm.nih.gov/pubmed/36420140
http://dx.doi.org/10.3389/fcell.2022.983097
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author Ferguson, Kirsty M.
Blin, Carla
Alfazema, Neza
Gangoso, Ester
Pollard, Steven M.
Marques-Torrejon, Maria Angeles
author_facet Ferguson, Kirsty M.
Blin, Carla
Alfazema, Neza
Gangoso, Ester
Pollard, Steven M.
Marques-Torrejon, Maria Angeles
author_sort Ferguson, Kirsty M.
collection PubMed
description Patients with glioblastoma (GBM) face a dismal prognosis. GBMs are driven by glioblastoma stem cells (GSCs) that display a neural stem cell (NSC)-like phenotype. These glioblastoma stem cells are often in a quiescent state that evades current therapies, namely debulking surgery and chemo/radiotherapy. Leucine-rich repeats and immunoglobulin-like domains (LRIG) proteins have been implicated as regulators of growth factor signalling across many tissue stem cells. Lrig1 is highly expressed in gliomas and importantly, polymorphisms have been identified that are risk alleles for patients with GBM, which suggests some functional role in gliomagenesis. We previously reported that Lrig1 is a gatekeeper of quiescence exit in adult mouse neural stem cells, suppressing epidermal growth factor receptor signalling prior to cell cycle re-entry. Here, we perform gain- and loss-of-function studies to understand the function of Lrig1 in glioblastoma stem cells. Using a novel mouse glioblastoma stem cell model, we show that genetic ablation of Lrig1 in cultured GBM stem cells results in higher proliferation and loss of quiescence. In vivo, mice transplanted with glioblastoma stem cells lacking Lrig1 display lower survival compared to Lrig1 WT glioblastoma stem cells, with tumours displaying increased proportions of proliferative cells and reduced quiescent subpopulations. In contrast, Lrig1 overexpression in mouse glioblastoma stem cells results in enhanced quiescence and reduced proliferation, with impaired tumour formation upon orthotopic transplantation. Mechanistically, we find that Lrig1-null cells have a deficiency in BMP signalling responses that may underlie their lack of responsiveness to quiescence cues in vivo. These findings highlight important roles for Lrig1 in controlling responsiveness to both epidermal growth factor receptor and BMPR signalling, and hence the proportions of quiescent and proliferative subpopulations in GBMs.
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spelling pubmed-96774542022-11-22 Lrig1 regulates the balance between proliferation and quiescence in glioblastoma stem cells Ferguson, Kirsty M. Blin, Carla Alfazema, Neza Gangoso, Ester Pollard, Steven M. Marques-Torrejon, Maria Angeles Front Cell Dev Biol Cell and Developmental Biology Patients with glioblastoma (GBM) face a dismal prognosis. GBMs are driven by glioblastoma stem cells (GSCs) that display a neural stem cell (NSC)-like phenotype. These glioblastoma stem cells are often in a quiescent state that evades current therapies, namely debulking surgery and chemo/radiotherapy. Leucine-rich repeats and immunoglobulin-like domains (LRIG) proteins have been implicated as regulators of growth factor signalling across many tissue stem cells. Lrig1 is highly expressed in gliomas and importantly, polymorphisms have been identified that are risk alleles for patients with GBM, which suggests some functional role in gliomagenesis. We previously reported that Lrig1 is a gatekeeper of quiescence exit in adult mouse neural stem cells, suppressing epidermal growth factor receptor signalling prior to cell cycle re-entry. Here, we perform gain- and loss-of-function studies to understand the function of Lrig1 in glioblastoma stem cells. Using a novel mouse glioblastoma stem cell model, we show that genetic ablation of Lrig1 in cultured GBM stem cells results in higher proliferation and loss of quiescence. In vivo, mice transplanted with glioblastoma stem cells lacking Lrig1 display lower survival compared to Lrig1 WT glioblastoma stem cells, with tumours displaying increased proportions of proliferative cells and reduced quiescent subpopulations. In contrast, Lrig1 overexpression in mouse glioblastoma stem cells results in enhanced quiescence and reduced proliferation, with impaired tumour formation upon orthotopic transplantation. Mechanistically, we find that Lrig1-null cells have a deficiency in BMP signalling responses that may underlie their lack of responsiveness to quiescence cues in vivo. These findings highlight important roles for Lrig1 in controlling responsiveness to both epidermal growth factor receptor and BMPR signalling, and hence the proportions of quiescent and proliferative subpopulations in GBMs. Frontiers Media S.A. 2022-10-26 /pmc/articles/PMC9677454/ /pubmed/36420140 http://dx.doi.org/10.3389/fcell.2022.983097 Text en Copyright © 2022 Ferguson, Blin, Alfazema, Gangoso, Pollard and Marques-Torrejon. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Ferguson, Kirsty M.
Blin, Carla
Alfazema, Neza
Gangoso, Ester
Pollard, Steven M.
Marques-Torrejon, Maria Angeles
Lrig1 regulates the balance between proliferation and quiescence in glioblastoma stem cells
title Lrig1 regulates the balance between proliferation and quiescence in glioblastoma stem cells
title_full Lrig1 regulates the balance between proliferation and quiescence in glioblastoma stem cells
title_fullStr Lrig1 regulates the balance between proliferation and quiescence in glioblastoma stem cells
title_full_unstemmed Lrig1 regulates the balance between proliferation and quiescence in glioblastoma stem cells
title_short Lrig1 regulates the balance between proliferation and quiescence in glioblastoma stem cells
title_sort lrig1 regulates the balance between proliferation and quiescence in glioblastoma stem cells
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677454/
https://www.ncbi.nlm.nih.gov/pubmed/36420140
http://dx.doi.org/10.3389/fcell.2022.983097
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