Protocol to measure end resection intermediates at sequence-specific DNA double-strand breaks by quantitative polymerase chain reaction using ER-AsiSI U2OS cells

DNA end resection is a critical step in the homologous recombination pathway of repairing DNA double-strand breaks (DSBs) that can be visualized in cells by detecting the generation of single-stranded DNA (ssDNA) intermediates formed during the resection of the DSBs. Here, we describe quantitative p...

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Detalles Bibliográficos
Autores principales: Sharma, Ajit K., Fitieh, Amira Mohammed, Hafez Ali, Jana Yasser, Ismail, Ismail Hassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678777/
https://www.ncbi.nlm.nih.gov/pubmed/36595899
http://dx.doi.org/10.1016/j.xpro.2022.101861

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678777/
https://www.ncbi.nlm.nih.gov/pubmed/36595899
http://dx.doi.org/10.1016/j.xpro.2022.101861

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