Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay

To understand how potential gene manipulations affect in vitro microglia, we provide a set of short protocols to evaluate microglia identity and function. We detail steps for immunostaining to determine microglia identity. We describe three functional assays for microglia: phagocytosis, calcium resp...

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Detalles Bibliográficos
Autores principales: Hübschmann, Verena, Korkut-Demirbaş, Medina, Siegert, Sandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678782/
https://www.ncbi.nlm.nih.gov/pubmed/36595902
http://dx.doi.org/10.1016/j.xpro.2022.101866

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678782/
https://www.ncbi.nlm.nih.gov/pubmed/36595902
http://dx.doi.org/10.1016/j.xpro.2022.101866

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