Protocol for gene characterization in Aspergillus niger using 5S rRNA-CRISPR-Cas9-mediated Tet-on inducible promoter exchange

This protocol presents an efficient genetic strategy to investigate gene function in the fungus Aspergillus niger. We combined 5S rRNA-CRISPR-Cas9 technology with Tet-on gene switch to generate conditional-expression mutants via precisely replacing native promoter with inducible promoter. We describ...

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Detalles Bibliográficos
Autores principales: Zheng, Xiaomei, Cairns, Timothy, Zheng, Ping, Meyer, Vera, Sun, Jibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678785/
https://www.ncbi.nlm.nih.gov/pubmed/36595926
http://dx.doi.org/10.1016/j.xpro.2022.101838

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678785/
https://www.ncbi.nlm.nih.gov/pubmed/36595926
http://dx.doi.org/10.1016/j.xpro.2022.101838

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