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Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments

The exopolysaccharides (EPS) produced by the edible medicinal fungus Monascus purpureus (EMP) become the center of growing interest due to their techno-functional properties and their numerous applications in the food industries; however, the low EPS yields limit its application. In this study, the...

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Autores principales: Xie, Liuming, Wang, Gang, Xie, Jianhua, Chen, XianXiang, Xie, Jiayan, Shi, Xiaoyi, Huang, Zhibing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678808/
https://www.ncbi.nlm.nih.gov/pubmed/36425595
http://dx.doi.org/10.1016/j.crfs.2022.11.011
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author Xie, Liuming
Wang, Gang
Xie, Jianhua
Chen, XianXiang
Xie, Jiayan
Shi, Xiaoyi
Huang, Zhibing
author_facet Xie, Liuming
Wang, Gang
Xie, Jianhua
Chen, XianXiang
Xie, Jiayan
Shi, Xiaoyi
Huang, Zhibing
author_sort Xie, Liuming
collection PubMed
description The exopolysaccharides (EPS) produced by the edible medicinal fungus Monascus purpureus (EMP) become the center of growing interest due to their techno-functional properties and their numerous applications in the food industries; however, the low EPS yields limit its application. In this study, the effect of genistein supplementation on the production, rheological and antioxidant properties of EPS by M. purpureus and its biosynthesis mechanism were explored. The results indicated that the addition of genistein (3 g/L) generated a 110% and 59% increase in the maximum mycelial biomass and EPS yield, respectively. The genistein supplementation group (G-EMP) had higher molar percentages of Xyl and Man, and significantly decreased molecule weight and particle size of EPS, which resulted in stronger antioxidant effect and cell growth promotion. Rheological analysis showed that both EMP and G-EMP demonstrated pseudoplastic fluid behavior and G-EMP exhibited strong gel-like elastic behavior (G' > G"). Furthermore, genistein not only facilitated the production of EPS by regulating cell membrane permeability, enhancing cellular respiratory metabolism and monosaccharide precursor synthesis pathways, and enhancing antioxidant enzyme activity to reduce oxidative stress damage, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways. These findings expand the application of M. purpureus resources and provide a paradigm for future study of the structural and functional characteristics of EPS.
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spelling pubmed-96788082022-11-23 Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments Xie, Liuming Wang, Gang Xie, Jianhua Chen, XianXiang Xie, Jiayan Shi, Xiaoyi Huang, Zhibing Curr Res Food Sci Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu The exopolysaccharides (EPS) produced by the edible medicinal fungus Monascus purpureus (EMP) become the center of growing interest due to their techno-functional properties and their numerous applications in the food industries; however, the low EPS yields limit its application. In this study, the effect of genistein supplementation on the production, rheological and antioxidant properties of EPS by M. purpureus and its biosynthesis mechanism were explored. The results indicated that the addition of genistein (3 g/L) generated a 110% and 59% increase in the maximum mycelial biomass and EPS yield, respectively. The genistein supplementation group (G-EMP) had higher molar percentages of Xyl and Man, and significantly decreased molecule weight and particle size of EPS, which resulted in stronger antioxidant effect and cell growth promotion. Rheological analysis showed that both EMP and G-EMP demonstrated pseudoplastic fluid behavior and G-EMP exhibited strong gel-like elastic behavior (G' > G"). Furthermore, genistein not only facilitated the production of EPS by regulating cell membrane permeability, enhancing cellular respiratory metabolism and monosaccharide precursor synthesis pathways, and enhancing antioxidant enzyme activity to reduce oxidative stress damage, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways. These findings expand the application of M. purpureus resources and provide a paradigm for future study of the structural and functional characteristics of EPS. Elsevier 2022-11-14 /pmc/articles/PMC9678808/ /pubmed/36425595 http://dx.doi.org/10.1016/j.crfs.2022.11.011 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu
Xie, Liuming
Wang, Gang
Xie, Jianhua
Chen, XianXiang
Xie, Jiayan
Shi, Xiaoyi
Huang, Zhibing
Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments
title Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments
title_full Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments
title_fullStr Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments
title_full_unstemmed Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments
title_short Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments
title_sort enhancement of functional activity and biosynthesis of exopolysaccharides in monascus purpureus by genistein treatments
topic Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678808/
https://www.ncbi.nlm.nih.gov/pubmed/36425595
http://dx.doi.org/10.1016/j.crfs.2022.11.011
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