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Labeling and analyzing lipid droplets in mouse muscle stem cells

Lipid droplets are emerging as an important and dynamic organelle whose metabolism controls stem cell behavior. Here we present a comprehensive protocol to visualize and quantify these organelles in mouse muscle satellite cells (MuSCs). This protocol includes steps for BODIPY/LipidSpot610 staining o...

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Detalles Bibliográficos
Autores principales: Chen, Jingjuan, Yue, Feng, Kuang, Shihuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679676/
https://www.ncbi.nlm.nih.gov/pubmed/36595920
http://dx.doi.org/10.1016/j.xpro.2022.101849