Labeling and analyzing lipid droplets in mouse muscle stem cells

Lipid droplets are emerging as an important and dynamic organelle whose metabolism controls stem cell behavior. Here we present a comprehensive protocol to visualize and quantify these organelles in mouse muscle satellite cells (MuSCs). This protocol includes steps for BODIPY/LipidSpot610 staining o...

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Detalles Bibliográficos
Autores principales: Chen, Jingjuan, Yue, Feng, Kuang, Shihuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679676/
https://www.ncbi.nlm.nih.gov/pubmed/36595920
http://dx.doi.org/10.1016/j.xpro.2022.101849
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author Chen, Jingjuan
Yue, Feng
Kuang, Shihuan
author_facet Chen, Jingjuan
Yue, Feng
Kuang, Shihuan
author_sort Chen, Jingjuan
collection PubMed
description Lipid droplets are emerging as an important and dynamic organelle whose metabolism controls stem cell behavior. Here we present a comprehensive protocol to visualize and quantify these organelles in mouse muscle satellite cells (MuSCs). This protocol includes steps for BODIPY/LipidSpot610 staining of freshly isolated MuSCs, in vitro cultured myoblasts, and single myofibers to label lipid droplets and subsequent analysis and quantification of fluorescence signals. This protocol can be modified to stain lipid droplets in other cell types of interest. For complete details on the use and execution of this protocol, please refer to Yue et al. (2022).(1)
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spelling pubmed-96796762022-11-23 Labeling and analyzing lipid droplets in mouse muscle stem cells Chen, Jingjuan Yue, Feng Kuang, Shihuan STAR Protoc Protocol Lipid droplets are emerging as an important and dynamic organelle whose metabolism controls stem cell behavior. Here we present a comprehensive protocol to visualize and quantify these organelles in mouse muscle satellite cells (MuSCs). This protocol includes steps for BODIPY/LipidSpot610 staining of freshly isolated MuSCs, in vitro cultured myoblasts, and single myofibers to label lipid droplets and subsequent analysis and quantification of fluorescence signals. This protocol can be modified to stain lipid droplets in other cell types of interest. For complete details on the use and execution of this protocol, please refer to Yue et al. (2022).(1) Elsevier 2022-11-19 /pmc/articles/PMC9679676/ /pubmed/36595920 http://dx.doi.org/10.1016/j.xpro.2022.101849 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chen, Jingjuan
Yue, Feng
Kuang, Shihuan
Labeling and analyzing lipid droplets in mouse muscle stem cells
title Labeling and analyzing lipid droplets in mouse muscle stem cells
title_full Labeling and analyzing lipid droplets in mouse muscle stem cells
title_fullStr Labeling and analyzing lipid droplets in mouse muscle stem cells
title_full_unstemmed Labeling and analyzing lipid droplets in mouse muscle stem cells
title_short Labeling and analyzing lipid droplets in mouse muscle stem cells
title_sort labeling and analyzing lipid droplets in mouse muscle stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679676/
https://www.ncbi.nlm.nih.gov/pubmed/36595920
http://dx.doi.org/10.1016/j.xpro.2022.101849
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AT kuangshihuan labelingandanalyzinglipiddropletsinmousemusclestemcells

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