ACtivE: Assembly and CRISPR-Targeted in Vivo Editing for Yeast Genome Engineering Using Minimum Reagents and Time

[Image: see text] Thanks to its sophistication, the CRISPR/Cas system has been a widely used yeast genome editing method. However, CRISPR methods generally rely on preassembled DNAs and extra cloning steps to deliver gRNA, Cas protein, and donor DNA. These laborious steps might hinder its usefulness...

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Detalles Bibliográficos
Autores principales: Malcı, Koray, Jonguitud-Borrego, Nestor, van der Straten Waillet, Hugo, Puodžiu̅naitė, Urtė, Johnston, Emily J., Rosser, Susan J., Rios-Solis, Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680028/
https://www.ncbi.nlm.nih.gov/pubmed/36252276
http://dx.doi.org/10.1021/acssynbio.2c00175

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680028/
https://www.ncbi.nlm.nih.gov/pubmed/36252276
http://dx.doi.org/10.1021/acssynbio.2c00175

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