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Probing DNA-protein interactions using single-molecule diffusivity contrast

Single-molecule fluorescence investigations of protein-nucleic acid interactions require robust means to identify the binding state of individual substrate molecules in real time. Here, we show that diffusivity contrast, widely used in fluorescence correlation spectroscopy at the ensemble level and...

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Detalles Bibliográficos
Autores principales: Wilson, Hugh, Lee, Miles, Wang, Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680706/
https://www.ncbi.nlm.nih.gov/pubmed/36425309
http://dx.doi.org/10.1016/j.bpr.2021.100009