A review of TNP-ATP in protein binding studies: benefits and pitfalls

We review 50 years of use of 2′,3′-O-trinitrophenyl (TNP)-ATP, a fluorescently tagged ATP analog. It has been extensively used to detect binding interactions of ATP to proteins and to measure parameters of those interactions such as the dissociation constant, K(d), or inhibitor dissociation constant...

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Detalles Bibliográficos
Autores principales: Woodbury, Dixon J., Whitt, Emily Campbell, Coffman, Robert E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680771/
https://www.ncbi.nlm.nih.gov/pubmed/36425312
http://dx.doi.org/10.1016/j.bpr.2021.100012

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680771/
https://www.ncbi.nlm.nih.gov/pubmed/36425312
http://dx.doi.org/10.1016/j.bpr.2021.100012

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