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A review of TNP-ATP in protein binding studies: benefits and pitfalls
We review 50 years of use of 2′,3′-O-trinitrophenyl (TNP)-ATP, a fluorescently tagged ATP analog. It has been extensively used to detect binding interactions of ATP to proteins and to measure parameters of those interactions such as the dissociation constant, K(d), or inhibitor dissociation constant...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680771/ https://www.ncbi.nlm.nih.gov/pubmed/36425312 http://dx.doi.org/10.1016/j.bpr.2021.100012 |