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Simultaneous depletion of RB, RBL1 and RBL2 affects endoderm differentiation of human embryonic stem cells
RB is a well-known cell cycle regulator controlling the G1 checkpoint. Previous reports have suggested that it can influence cell fate decisions not only by regulating cell proliferation and survival but also by interacting with transcription factors and epigenetic modifiers. However, the functional...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9681086/ https://www.ncbi.nlm.nih.gov/pubmed/36413521 http://dx.doi.org/10.1371/journal.pone.0269122 |
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author | Nakanoh, Shota Kadiwala, Juned Pinte, Laetitia Morell, Carola Maria Lenaerts, An-Sofie Vallier, Ludovic |
author_facet | Nakanoh, Shota Kadiwala, Juned Pinte, Laetitia Morell, Carola Maria Lenaerts, An-Sofie Vallier, Ludovic |
author_sort | Nakanoh, Shota |
collection | PubMed |
description | RB is a well-known cell cycle regulator controlling the G1 checkpoint. Previous reports have suggested that it can influence cell fate decisions not only by regulating cell proliferation and survival but also by interacting with transcription factors and epigenetic modifiers. However, the functional redundancy of RB family proteins (RB, RBL1 and RBL2) renders it difficult to investigate their roles during early development, especially in human. Here, we address this problem by generating human embryonic stem cells lacking RB family proteins. To achieve this goal, we first introduced frameshift mutations in RBL1 and RBL2 genes using the CRISPR/Cas9 technology, and then integrated the shRNA-expression cassette to knockdown RB upon tetracycline treatment. The resulting RBL1/2_dKO+RB_iKD cells remain pluripotent and efficiently differentiate into the primary germ layers in vitro even in the absence of the RB family proteins. In contrast, we observed that subsequent differentiation into foregut endoderm was impaired without the expression of RB, RBL1 and RBL2. Thus, it is suggested that RB proteins are dispensable for the maintenance and acquisition of cell identities during early development, but they are essential to generate advanced derivatives after the formation of primary germ layers. These results also indicate that our RBL1/2_dKO+RB_iKD cell lines are useful to depict the detailed molecular roles of RB family proteins in the maintenance and generation of various cell types accessible from human pluripotent stem cells. |
format | Online Article Text |
id | pubmed-9681086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-96810862022-11-23 Simultaneous depletion of RB, RBL1 and RBL2 affects endoderm differentiation of human embryonic stem cells Nakanoh, Shota Kadiwala, Juned Pinte, Laetitia Morell, Carola Maria Lenaerts, An-Sofie Vallier, Ludovic PLoS One Research Article RB is a well-known cell cycle regulator controlling the G1 checkpoint. Previous reports have suggested that it can influence cell fate decisions not only by regulating cell proliferation and survival but also by interacting with transcription factors and epigenetic modifiers. However, the functional redundancy of RB family proteins (RB, RBL1 and RBL2) renders it difficult to investigate their roles during early development, especially in human. Here, we address this problem by generating human embryonic stem cells lacking RB family proteins. To achieve this goal, we first introduced frameshift mutations in RBL1 and RBL2 genes using the CRISPR/Cas9 technology, and then integrated the shRNA-expression cassette to knockdown RB upon tetracycline treatment. The resulting RBL1/2_dKO+RB_iKD cells remain pluripotent and efficiently differentiate into the primary germ layers in vitro even in the absence of the RB family proteins. In contrast, we observed that subsequent differentiation into foregut endoderm was impaired without the expression of RB, RBL1 and RBL2. Thus, it is suggested that RB proteins are dispensable for the maintenance and acquisition of cell identities during early development, but they are essential to generate advanced derivatives after the formation of primary germ layers. These results also indicate that our RBL1/2_dKO+RB_iKD cell lines are useful to depict the detailed molecular roles of RB family proteins in the maintenance and generation of various cell types accessible from human pluripotent stem cells. Public Library of Science 2022-11-22 /pmc/articles/PMC9681086/ /pubmed/36413521 http://dx.doi.org/10.1371/journal.pone.0269122 Text en © 2022 Nakanoh et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Nakanoh, Shota Kadiwala, Juned Pinte, Laetitia Morell, Carola Maria Lenaerts, An-Sofie Vallier, Ludovic Simultaneous depletion of RB, RBL1 and RBL2 affects endoderm differentiation of human embryonic stem cells |
title | Simultaneous depletion of RB, RBL1 and RBL2 affects endoderm differentiation of human embryonic stem cells |
title_full | Simultaneous depletion of RB, RBL1 and RBL2 affects endoderm differentiation of human embryonic stem cells |
title_fullStr | Simultaneous depletion of RB, RBL1 and RBL2 affects endoderm differentiation of human embryonic stem cells |
title_full_unstemmed | Simultaneous depletion of RB, RBL1 and RBL2 affects endoderm differentiation of human embryonic stem cells |
title_short | Simultaneous depletion of RB, RBL1 and RBL2 affects endoderm differentiation of human embryonic stem cells |
title_sort | simultaneous depletion of rb, rbl1 and rbl2 affects endoderm differentiation of human embryonic stem cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9681086/ https://www.ncbi.nlm.nih.gov/pubmed/36413521 http://dx.doi.org/10.1371/journal.pone.0269122 |
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