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Detection of concentration-dependent conformational changes in SARS-CoV-2 nucleoprotein by agarose native gel electrophoresis

The nucleoprotein (NP) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is abundantly expressed during infection, making it a diagnostic target protein. We analyzed the structure of the NP in solution using a recombinant protein produced in E. coli. A codon-optimized Profinity eXact™-...

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Detalles Bibliográficos
Autores principales: Sato, Ryo, Tomioka, Yui, Sakuma, Chiaki, Nakagawa, Masataka, Kurosawa, Yasunori, Shiba, Kohei, Arakawa, Tsutomu, Akuta, Teruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9681993/
https://www.ncbi.nlm.nih.gov/pubmed/36427555
http://dx.doi.org/10.1016/j.ab.2022.114995