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A general approach for stabilizing nanobodies for intracellular expression

Conventional antibodies and their derived fragments are difficult to deploy against intracellular targets in live cells, due to their bulk and structural complexity. Nanobodies provide an alternative modality, with well-documented examples of intracellular expression. Despite their promise as intrac...

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Detalles Bibliográficos
Autores principales: Dingus, John G, Tang, Jonathan CY, Amamoto, Ryoji, Wallick, Grace K, Cepko, Constance L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683787/
https://www.ncbi.nlm.nih.gov/pubmed/36416528
http://dx.doi.org/10.7554/eLife.68253