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Microsecond melting and revitrification of cryo samples with a correlative light-electron microscopy approach

We have recently introduced a novel approach to time-resolved cryo-electron microscopy (cryo-EM) that affords microsecond time resolution. It involves melting a cryo sample with a laser beam to allow dynamics of the embedded particles to occur. Once the laser beam is switched off, the sample revitri...

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Detalles Bibliográficos
Autores principales: Bongiovanni, Gabriele, Harder, Oliver F., Drabbels, Marcel, Lorenz, Ulrich J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9685559/
https://www.ncbi.nlm.nih.gov/pubmed/36438663
http://dx.doi.org/10.3389/fmolb.2022.1044509