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A simple and rapid technique of template preparation for PCR

Many techniques have been developed for extracting DNA, but most are often complex, time-consuming, and/or expensive. In this study, we describe a simple, rapid and cost-effective technique for preparing DNA template for PCR. This technique involves 0.1 M potassium hydroxide treatment at 100°C for 1...

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Detalles Bibliográficos
Autores principales: Liu, Yunyun, Chen, Jia, Cheng, Yi, Li, Yi, Li, Xinwen, Zhang, Zhengbing, Xu, Xiumei, Lin, Yufeng, Xu, Jianping, Li, Zhimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686307/
https://www.ncbi.nlm.nih.gov/pubmed/36439815
http://dx.doi.org/10.3389/fmicb.2022.1024827