Cargando…
A simple and rapid technique of template preparation for PCR
Many techniques have been developed for extracting DNA, but most are often complex, time-consuming, and/or expensive. In this study, we describe a simple, rapid and cost-effective technique for preparing DNA template for PCR. This technique involves 0.1 M potassium hydroxide treatment at 100°C for 1...
Autores principales: | Liu, Yunyun, Chen, Jia, Cheng, Yi, Li, Yi, Li, Xinwen, Zhang, Zhengbing, Xu, Xiumei, Lin, Yufeng, Xu, Jianping, Li, Zhimin |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686307/ https://www.ncbi.nlm.nih.gov/pubmed/36439815 http://dx.doi.org/10.3389/fmicb.2022.1024827 |
Ejemplares similares
-
Development of a Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Phytopythium vexans
por: Wang, Tuhong, et al.
Publicado: (2021) -
Rapid Detection of Fusarium oxysporum Using Insulated Isothermal PCR and a Rapid, Simple DNA Preparation Protocol
por: Chang, Tsai-De, et al.
Publicado: (2022) -
Rapid detection of Mucorales based on recombinase polymerase amplification and real-time PCR
por: Xu, Rui, et al.
Publicado: (2023) -
Bacterial and fungal communities within and among geographic samples of the hemp pest Psylliodes attenuata from China
por: Guo, Litao, et al.
Publicado: (2022) -
Simple Sample Preparation Method for Direct Microbial Identification and Susceptibility Testing From Positive Blood Cultures
por: Pan, Hong-wei, et al.
Publicado: (2018)