Improving Precise Genome Editing Using Donor DNA/gRNA Hybrid Duplex Generated by Complementary Bases

In precise genome editing, site-specific DNA double-strand breaks (DSBs) induced by the CRISPR/Cas9 system are repaired via homology-directed repair (HDR) using exogenous donor DNA templates. However, the low efficiency of HDR-mediated genome editing is a barrier to widespread use. In this study, we...

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Detalles Bibliográficos
Autores principales: Aiba, Wataru, Amai, Takamitsu, Ueda, Mitsuyoshi, Kuroda, Kouichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687273/
https://www.ncbi.nlm.nih.gov/pubmed/36358971
http://dx.doi.org/10.3390/biom12111621

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687273/
https://www.ncbi.nlm.nih.gov/pubmed/36358971
http://dx.doi.org/10.3390/biom12111621

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