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Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications

Routine assessment of sperm DNA integrity involves the time-consuming and complex process of staining sperm chromatin. Here, we report a Raman spectroscopy method combined with extended multiplicative signal correction (EMSC) for the extraction of characteristic fingerprints of DNA-intact and DNA-da...

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Detalles Bibliográficos
Autores principales: Du, Shengrong, Zhang, Qun, Guan, Haohao, Chen, Guannan, Wang, Sisi, Sun, Yan, Li, Yuling, Chen, Rong, He, Youwu, Huang, Zufang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688089/
https://www.ncbi.nlm.nih.gov/pubmed/36421168
http://dx.doi.org/10.3390/bios12111051