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Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications

Routine assessment of sperm DNA integrity involves the time-consuming and complex process of staining sperm chromatin. Here, we report a Raman spectroscopy method combined with extended multiplicative signal correction (EMSC) for the extraction of characteristic fingerprints of DNA-intact and DNA-da...

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Autores principales: Du, Shengrong, Zhang, Qun, Guan, Haohao, Chen, Guannan, Wang, Sisi, Sun, Yan, Li, Yuling, Chen, Rong, He, Youwu, Huang, Zufang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688089/
https://www.ncbi.nlm.nih.gov/pubmed/36421168
http://dx.doi.org/10.3390/bios12111051
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author Du, Shengrong
Zhang, Qun
Guan, Haohao
Chen, Guannan
Wang, Sisi
Sun, Yan
Li, Yuling
Chen, Rong
He, Youwu
Huang, Zufang
author_facet Du, Shengrong
Zhang, Qun
Guan, Haohao
Chen, Guannan
Wang, Sisi
Sun, Yan
Li, Yuling
Chen, Rong
He, Youwu
Huang, Zufang
author_sort Du, Shengrong
collection PubMed
description Routine assessment of sperm DNA integrity involves the time-consuming and complex process of staining sperm chromatin. Here, we report a Raman spectroscopy method combined with extended multiplicative signal correction (EMSC) for the extraction of characteristic fingerprints of DNA-intact and DNA-damaged sperm cells directly on glass slides. Raman results of sperm cell DNA integrity on glass substrates were validated one-to-one with clinical sperm cell staining. Although the overall Raman spectral pattern showed considerable similarity between DNA-damaged and DNA-intact sperm cells, differences in specific Raman spectral responses were observed. We then employed and compared multivariate statistical analysis based on principal component analysis-linear discriminant analysis (PCA-LDA) and partial least-squares-discriminant analysis (PLS-DA), and the classifications were validated by leave-one-out-cross-validation (LOOCV) and k-fold cross-validation methods. In comparison, the PLS-DA model showed relatively better results in terms of diagnostic sensitivity, specificity, and the classification rate between the sperm DNA damaged group and the DNA intact group. Our results demonstrate the potential of Raman based label-free DNA assessment of sperm cell on glass substrates as a simple method toward clinical applications.
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spelling pubmed-96880892022-11-25 Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications Du, Shengrong Zhang, Qun Guan, Haohao Chen, Guannan Wang, Sisi Sun, Yan Li, Yuling Chen, Rong He, Youwu Huang, Zufang Biosensors (Basel) Article Routine assessment of sperm DNA integrity involves the time-consuming and complex process of staining sperm chromatin. Here, we report a Raman spectroscopy method combined with extended multiplicative signal correction (EMSC) for the extraction of characteristic fingerprints of DNA-intact and DNA-damaged sperm cells directly on glass slides. Raman results of sperm cell DNA integrity on glass substrates were validated one-to-one with clinical sperm cell staining. Although the overall Raman spectral pattern showed considerable similarity between DNA-damaged and DNA-intact sperm cells, differences in specific Raman spectral responses were observed. We then employed and compared multivariate statistical analysis based on principal component analysis-linear discriminant analysis (PCA-LDA) and partial least-squares-discriminant analysis (PLS-DA), and the classifications were validated by leave-one-out-cross-validation (LOOCV) and k-fold cross-validation methods. In comparison, the PLS-DA model showed relatively better results in terms of diagnostic sensitivity, specificity, and the classification rate between the sperm DNA damaged group and the DNA intact group. Our results demonstrate the potential of Raman based label-free DNA assessment of sperm cell on glass substrates as a simple method toward clinical applications. MDPI 2022-11-21 /pmc/articles/PMC9688089/ /pubmed/36421168 http://dx.doi.org/10.3390/bios12111051 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Du, Shengrong
Zhang, Qun
Guan, Haohao
Chen, Guannan
Wang, Sisi
Sun, Yan
Li, Yuling
Chen, Rong
He, Youwu
Huang, Zufang
Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications
title Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications
title_full Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications
title_fullStr Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications
title_full_unstemmed Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications
title_short Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications
title_sort micro-raman analysis of sperm cells on glass slide: potential label-free assessment of sperm dna toward clinical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688089/
https://www.ncbi.nlm.nih.gov/pubmed/36421168
http://dx.doi.org/10.3390/bios12111051
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