Method for the Isolation of “RNA-seq-Quality” RNA from Human Intervertebral Discs after Mortar and Pestle Homogenization

The problem of isolating high-quality total RNA from intervertebral discs has no recognized solution yet. This is due to the extremely low content of live cells in the samples and the voluminous intercellular matrix. A variety of published protocols focused on isolating RNA from articular cartilage...

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Detalles Bibliográficos
Autores principales: Ivanov, Artemii A., Leonova, Olga N., Wiebe, Daniil S., Krutko, Alexsandr V., Gridina, Mariya M., Fishman, Veniamin S., Aulchenko, Yurii S., Tsepilov, Yakov A., Golubeva, Tatiana S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688557/
https://www.ncbi.nlm.nih.gov/pubmed/36429007
http://dx.doi.org/10.3390/cells11223578

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688557/
https://www.ncbi.nlm.nih.gov/pubmed/36429007
http://dx.doi.org/10.3390/cells11223578

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