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Distinct Dynamics of Migratory Response to PD-1 and CTLA-4 Blockade Reveals New Mechanistic Insights for Potential T-Cell Reinvigoration following Immune Checkpoint Blockade

Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell death protein 1 (PD-1), two clinically relevant targets for the immunotherapy of cancer, are negative regulators of T-cell activation and migration. Optimizing the therapeutic response to CTLA-4 and PD-1 blockade calls for a mo...

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Autores principales: Safaeifard, Fateme, Goliaei, Bahram, Aref, Amir R., Foroughmand-Araabi, Mohammad-Hadi, Goliaei, Sama, Lorch, Jochen, Jenkins, Russell W., Barbie, David A., Shariatpanahi, Seyed Peyman, Rüegg, Curzio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688893/
https://www.ncbi.nlm.nih.gov/pubmed/36428963
http://dx.doi.org/10.3390/cells11223534
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author Safaeifard, Fateme
Goliaei, Bahram
Aref, Amir R.
Foroughmand-Araabi, Mohammad-Hadi
Goliaei, Sama
Lorch, Jochen
Jenkins, Russell W.
Barbie, David A.
Shariatpanahi, Seyed Peyman
Rüegg, Curzio
author_facet Safaeifard, Fateme
Goliaei, Bahram
Aref, Amir R.
Foroughmand-Araabi, Mohammad-Hadi
Goliaei, Sama
Lorch, Jochen
Jenkins, Russell W.
Barbie, David A.
Shariatpanahi, Seyed Peyman
Rüegg, Curzio
author_sort Safaeifard, Fateme
collection PubMed
description Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell death protein 1 (PD-1), two clinically relevant targets for the immunotherapy of cancer, are negative regulators of T-cell activation and migration. Optimizing the therapeutic response to CTLA-4 and PD-1 blockade calls for a more comprehensive insight into the coordinated function of these immune regulators. Mathematical modeling can be used to elucidate nonlinear tumor–immune interactions and highlight the underlying mechanisms to tackle the problem. Here, we investigated and statistically characterized the dynamics of T-cell migration as a measure of the functional response to these pathways. We used a previously developed three-dimensional organotypic culture of patient-derived tumor spheroids treated with anti-CTLA-4 and anti-PD-1 antibodies for this purpose. Experiment-based dynamical modeling revealed the delayed kinetics of PD-1 activation, which originates from the distinct characteristics of PD-1 and CTLA-4 regulation, and followed through with the modification of their contributions to immune modulation. The simulation results show good agreement with the tumor cell reduction and active immune cell count in each experiment. Our findings demonstrate that while PD-1 activation provokes a more exhaustive intracellular cascade within a mature tumor environment, the time-delayed kinetics of PD-1 activation outweighs its preeminence at the individual cell level and consequently confers a functional dominance to the CTLA-4 checkpoint. The proposed model explains the distinct immunostimulatory pattern of PD-1 and CTLA-4 blockade based on mechanisms involved in the regulation of their expression and may be useful for planning effective treatment schemes targeting PD-1 and CTLA-4 functions.
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spelling pubmed-96888932022-11-25 Distinct Dynamics of Migratory Response to PD-1 and CTLA-4 Blockade Reveals New Mechanistic Insights for Potential T-Cell Reinvigoration following Immune Checkpoint Blockade Safaeifard, Fateme Goliaei, Bahram Aref, Amir R. Foroughmand-Araabi, Mohammad-Hadi Goliaei, Sama Lorch, Jochen Jenkins, Russell W. Barbie, David A. Shariatpanahi, Seyed Peyman Rüegg, Curzio Cells Article Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell death protein 1 (PD-1), two clinically relevant targets for the immunotherapy of cancer, are negative regulators of T-cell activation and migration. Optimizing the therapeutic response to CTLA-4 and PD-1 blockade calls for a more comprehensive insight into the coordinated function of these immune regulators. Mathematical modeling can be used to elucidate nonlinear tumor–immune interactions and highlight the underlying mechanisms to tackle the problem. Here, we investigated and statistically characterized the dynamics of T-cell migration as a measure of the functional response to these pathways. We used a previously developed three-dimensional organotypic culture of patient-derived tumor spheroids treated with anti-CTLA-4 and anti-PD-1 antibodies for this purpose. Experiment-based dynamical modeling revealed the delayed kinetics of PD-1 activation, which originates from the distinct characteristics of PD-1 and CTLA-4 regulation, and followed through with the modification of their contributions to immune modulation. The simulation results show good agreement with the tumor cell reduction and active immune cell count in each experiment. Our findings demonstrate that while PD-1 activation provokes a more exhaustive intracellular cascade within a mature tumor environment, the time-delayed kinetics of PD-1 activation outweighs its preeminence at the individual cell level and consequently confers a functional dominance to the CTLA-4 checkpoint. The proposed model explains the distinct immunostimulatory pattern of PD-1 and CTLA-4 blockade based on mechanisms involved in the regulation of their expression and may be useful for planning effective treatment schemes targeting PD-1 and CTLA-4 functions. MDPI 2022-11-08 /pmc/articles/PMC9688893/ /pubmed/36428963 http://dx.doi.org/10.3390/cells11223534 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Safaeifard, Fateme
Goliaei, Bahram
Aref, Amir R.
Foroughmand-Araabi, Mohammad-Hadi
Goliaei, Sama
Lorch, Jochen
Jenkins, Russell W.
Barbie, David A.
Shariatpanahi, Seyed Peyman
Rüegg, Curzio
Distinct Dynamics of Migratory Response to PD-1 and CTLA-4 Blockade Reveals New Mechanistic Insights for Potential T-Cell Reinvigoration following Immune Checkpoint Blockade
title Distinct Dynamics of Migratory Response to PD-1 and CTLA-4 Blockade Reveals New Mechanistic Insights for Potential T-Cell Reinvigoration following Immune Checkpoint Blockade
title_full Distinct Dynamics of Migratory Response to PD-1 and CTLA-4 Blockade Reveals New Mechanistic Insights for Potential T-Cell Reinvigoration following Immune Checkpoint Blockade
title_fullStr Distinct Dynamics of Migratory Response to PD-1 and CTLA-4 Blockade Reveals New Mechanistic Insights for Potential T-Cell Reinvigoration following Immune Checkpoint Blockade
title_full_unstemmed Distinct Dynamics of Migratory Response to PD-1 and CTLA-4 Blockade Reveals New Mechanistic Insights for Potential T-Cell Reinvigoration following Immune Checkpoint Blockade
title_short Distinct Dynamics of Migratory Response to PD-1 and CTLA-4 Blockade Reveals New Mechanistic Insights for Potential T-Cell Reinvigoration following Immune Checkpoint Blockade
title_sort distinct dynamics of migratory response to pd-1 and ctla-4 blockade reveals new mechanistic insights for potential t-cell reinvigoration following immune checkpoint blockade
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688893/
https://www.ncbi.nlm.nih.gov/pubmed/36428963
http://dx.doi.org/10.3390/cells11223534
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