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Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol

Currently, the demand for low-calorie sweeteners has grown dramatically because consumers are more mindful of their health than they used to be. Therefore, bioproduction of low-calorie sweeteners from low-cost raw materials becomes a hot spot. In this study, a two-stage strategy was established to e...

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Detalles Bibliográficos
Autores principales: Li, Jin, Chen, Jiajun, Xu, Wei, Zhang, Wenli, Chen, Yeming, Mu, Wanmeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689084/
https://www.ncbi.nlm.nih.gov/pubmed/36429205
http://dx.doi.org/10.3390/foods11223613
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author Li, Jin
Chen, Jiajun
Xu, Wei
Zhang, Wenli
Chen, Yeming
Mu, Wanmeng
author_facet Li, Jin
Chen, Jiajun
Xu, Wei
Zhang, Wenli
Chen, Yeming
Mu, Wanmeng
author_sort Li, Jin
collection PubMed
description Currently, the demand for low-calorie sweeteners has grown dramatically because consumers are more mindful of their health than they used to be. Therefore, bioproduction of low-calorie sweeteners from low-cost raw materials becomes a hot spot. In this study, a two-stage strategy was established to efficiently utilize D-fructose from fruit and vegetable wastes. Firstly, ketose 3-epimerase was used to produce D-allulose from D-fructose of pear peels. Secondly, the residual D-fructose was converted to D-mannitol by the engineered strain co-expression of D-mannitol 2-dehydrogenase and formate dehydrogenase. Approximately 29.4% D-fructose of pear peels was converted to D-allulose. Subsequently, under optimal conditions (35 °C, pH 6.5, 1 mM Mn(2+), 2 g/L dry cells), almost all the residual D-fructose was transformed into D-mannitol with a 93.5% conversion rate. Eventually, from 1 kg fresh pear peel, it could produce 10.8 g of D-allulose and 24.6 g of D-mannitol. This bioprocess strategy provides a vital method to biosynthesize high-value functional sugars from low-cost biomass.
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spelling pubmed-96890842022-11-25 Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol Li, Jin Chen, Jiajun Xu, Wei Zhang, Wenli Chen, Yeming Mu, Wanmeng Foods Article Currently, the demand for low-calorie sweeteners has grown dramatically because consumers are more mindful of their health than they used to be. Therefore, bioproduction of low-calorie sweeteners from low-cost raw materials becomes a hot spot. In this study, a two-stage strategy was established to efficiently utilize D-fructose from fruit and vegetable wastes. Firstly, ketose 3-epimerase was used to produce D-allulose from D-fructose of pear peels. Secondly, the residual D-fructose was converted to D-mannitol by the engineered strain co-expression of D-mannitol 2-dehydrogenase and formate dehydrogenase. Approximately 29.4% D-fructose of pear peels was converted to D-allulose. Subsequently, under optimal conditions (35 °C, pH 6.5, 1 mM Mn(2+), 2 g/L dry cells), almost all the residual D-fructose was transformed into D-mannitol with a 93.5% conversion rate. Eventually, from 1 kg fresh pear peel, it could produce 10.8 g of D-allulose and 24.6 g of D-mannitol. This bioprocess strategy provides a vital method to biosynthesize high-value functional sugars from low-cost biomass. MDPI 2022-11-12 /pmc/articles/PMC9689084/ /pubmed/36429205 http://dx.doi.org/10.3390/foods11223613 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Jin
Chen, Jiajun
Xu, Wei
Zhang, Wenli
Chen, Yeming
Mu, Wanmeng
Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol
title Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol
title_full Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol
title_fullStr Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol
title_full_unstemmed Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol
title_short Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol
title_sort efficient utilization of fruit peels for the bioproduction of d-allulose and d-mannitol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689084/
https://www.ncbi.nlm.nih.gov/pubmed/36429205
http://dx.doi.org/10.3390/foods11223613
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