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Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol
Currently, the demand for low-calorie sweeteners has grown dramatically because consumers are more mindful of their health than they used to be. Therefore, bioproduction of low-calorie sweeteners from low-cost raw materials becomes a hot spot. In this study, a two-stage strategy was established to e...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689084/ https://www.ncbi.nlm.nih.gov/pubmed/36429205 http://dx.doi.org/10.3390/foods11223613 |
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author | Li, Jin Chen, Jiajun Xu, Wei Zhang, Wenli Chen, Yeming Mu, Wanmeng |
author_facet | Li, Jin Chen, Jiajun Xu, Wei Zhang, Wenli Chen, Yeming Mu, Wanmeng |
author_sort | Li, Jin |
collection | PubMed |
description | Currently, the demand for low-calorie sweeteners has grown dramatically because consumers are more mindful of their health than they used to be. Therefore, bioproduction of low-calorie sweeteners from low-cost raw materials becomes a hot spot. In this study, a two-stage strategy was established to efficiently utilize D-fructose from fruit and vegetable wastes. Firstly, ketose 3-epimerase was used to produce D-allulose from D-fructose of pear peels. Secondly, the residual D-fructose was converted to D-mannitol by the engineered strain co-expression of D-mannitol 2-dehydrogenase and formate dehydrogenase. Approximately 29.4% D-fructose of pear peels was converted to D-allulose. Subsequently, under optimal conditions (35 °C, pH 6.5, 1 mM Mn(2+), 2 g/L dry cells), almost all the residual D-fructose was transformed into D-mannitol with a 93.5% conversion rate. Eventually, from 1 kg fresh pear peel, it could produce 10.8 g of D-allulose and 24.6 g of D-mannitol. This bioprocess strategy provides a vital method to biosynthesize high-value functional sugars from low-cost biomass. |
format | Online Article Text |
id | pubmed-9689084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96890842022-11-25 Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol Li, Jin Chen, Jiajun Xu, Wei Zhang, Wenli Chen, Yeming Mu, Wanmeng Foods Article Currently, the demand for low-calorie sweeteners has grown dramatically because consumers are more mindful of their health than they used to be. Therefore, bioproduction of low-calorie sweeteners from low-cost raw materials becomes a hot spot. In this study, a two-stage strategy was established to efficiently utilize D-fructose from fruit and vegetable wastes. Firstly, ketose 3-epimerase was used to produce D-allulose from D-fructose of pear peels. Secondly, the residual D-fructose was converted to D-mannitol by the engineered strain co-expression of D-mannitol 2-dehydrogenase and formate dehydrogenase. Approximately 29.4% D-fructose of pear peels was converted to D-allulose. Subsequently, under optimal conditions (35 °C, pH 6.5, 1 mM Mn(2+), 2 g/L dry cells), almost all the residual D-fructose was transformed into D-mannitol with a 93.5% conversion rate. Eventually, from 1 kg fresh pear peel, it could produce 10.8 g of D-allulose and 24.6 g of D-mannitol. This bioprocess strategy provides a vital method to biosynthesize high-value functional sugars from low-cost biomass. MDPI 2022-11-12 /pmc/articles/PMC9689084/ /pubmed/36429205 http://dx.doi.org/10.3390/foods11223613 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Jin Chen, Jiajun Xu, Wei Zhang, Wenli Chen, Yeming Mu, Wanmeng Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol |
title | Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol |
title_full | Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol |
title_fullStr | Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol |
title_full_unstemmed | Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol |
title_short | Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol |
title_sort | efficient utilization of fruit peels for the bioproduction of d-allulose and d-mannitol |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689084/ https://www.ncbi.nlm.nih.gov/pubmed/36429205 http://dx.doi.org/10.3390/foods11223613 |
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