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Effects of Escherichia coli and Proteus mirabilis on the Growth and Aggregation of Calcium Oxalate Crystal under Microaerobic Conditions

Escherichia coli and Proteus mirabilis are common single- and polymicrobial urinary tract infections which can survive under various oxygen levels, including inside of stone matrices. Therefore, we aimed to investigate and compare the calcium oxalate monohydrate (COM) lithogenic activities including...

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Autores principales: Saelee, Krittaya, Lulitanond, Aroonlug, Sae-ung, Nattaya, Prasongwatana, Vitoon, Boonsiri, Patcharee, Tavichakorntrakool, Ratree
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689598/
https://www.ncbi.nlm.nih.gov/pubmed/36359494
http://dx.doi.org/10.3390/diagnostics12112651
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author Saelee, Krittaya
Lulitanond, Aroonlug
Sae-ung, Nattaya
Prasongwatana, Vitoon
Boonsiri, Patcharee
Tavichakorntrakool, Ratree
author_facet Saelee, Krittaya
Lulitanond, Aroonlug
Sae-ung, Nattaya
Prasongwatana, Vitoon
Boonsiri, Patcharee
Tavichakorntrakool, Ratree
author_sort Saelee, Krittaya
collection PubMed
description Escherichia coli and Proteus mirabilis are common single- and polymicrobial urinary tract infections which can survive under various oxygen levels, including inside of stone matrices. Therefore, we aimed to investigate and compare the calcium oxalate monohydrate (COM) lithogenic activities including COM crystal growth and aggregation under microaerobic conditions of E. coli and P. mirabilis isolated from the same stone matrix. The crystal growth was analyzed as the delta crystal area while the crystal aggregation was analyzed as the number of crystal aggregates. The results showed that compared to blank control, E. coli, P. mirabilis and the co-culture of E. coli and P. mirabilis were able to significantly promote COM crystal growth under microaerobic conditions. Interestingly, the delta crystal area in the co-culture under microaerobic conditions was larger than that of E. coli alone and P. mirabilis alone. In addition, only P. mirabilis alone and the co-culture were able to significantly increase COM aggregates. This study demonstrated that single- and co-culture of E. coli and P. mirabilis could promote COM crystal growth and aggregation under microaerobic conditions. The co-culture of E. coli and P. mirabilis may provide the combination effect on COM crystal interactions. The bacterial surfaces and the important effects on bacteria–crystal interactions should be further evaluated.
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spelling pubmed-96895982022-11-25 Effects of Escherichia coli and Proteus mirabilis on the Growth and Aggregation of Calcium Oxalate Crystal under Microaerobic Conditions Saelee, Krittaya Lulitanond, Aroonlug Sae-ung, Nattaya Prasongwatana, Vitoon Boonsiri, Patcharee Tavichakorntrakool, Ratree Diagnostics (Basel) Article Escherichia coli and Proteus mirabilis are common single- and polymicrobial urinary tract infections which can survive under various oxygen levels, including inside of stone matrices. Therefore, we aimed to investigate and compare the calcium oxalate monohydrate (COM) lithogenic activities including COM crystal growth and aggregation under microaerobic conditions of E. coli and P. mirabilis isolated from the same stone matrix. The crystal growth was analyzed as the delta crystal area while the crystal aggregation was analyzed as the number of crystal aggregates. The results showed that compared to blank control, E. coli, P. mirabilis and the co-culture of E. coli and P. mirabilis were able to significantly promote COM crystal growth under microaerobic conditions. Interestingly, the delta crystal area in the co-culture under microaerobic conditions was larger than that of E. coli alone and P. mirabilis alone. In addition, only P. mirabilis alone and the co-culture were able to significantly increase COM aggregates. This study demonstrated that single- and co-culture of E. coli and P. mirabilis could promote COM crystal growth and aggregation under microaerobic conditions. The co-culture of E. coli and P. mirabilis may provide the combination effect on COM crystal interactions. The bacterial surfaces and the important effects on bacteria–crystal interactions should be further evaluated. MDPI 2022-10-31 /pmc/articles/PMC9689598/ /pubmed/36359494 http://dx.doi.org/10.3390/diagnostics12112651 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saelee, Krittaya
Lulitanond, Aroonlug
Sae-ung, Nattaya
Prasongwatana, Vitoon
Boonsiri, Patcharee
Tavichakorntrakool, Ratree
Effects of Escherichia coli and Proteus mirabilis on the Growth and Aggregation of Calcium Oxalate Crystal under Microaerobic Conditions
title Effects of Escherichia coli and Proteus mirabilis on the Growth and Aggregation of Calcium Oxalate Crystal under Microaerobic Conditions
title_full Effects of Escherichia coli and Proteus mirabilis on the Growth and Aggregation of Calcium Oxalate Crystal under Microaerobic Conditions
title_fullStr Effects of Escherichia coli and Proteus mirabilis on the Growth and Aggregation of Calcium Oxalate Crystal under Microaerobic Conditions
title_full_unstemmed Effects of Escherichia coli and Proteus mirabilis on the Growth and Aggregation of Calcium Oxalate Crystal under Microaerobic Conditions
title_short Effects of Escherichia coli and Proteus mirabilis on the Growth and Aggregation of Calcium Oxalate Crystal under Microaerobic Conditions
title_sort effects of escherichia coli and proteus mirabilis on the growth and aggregation of calcium oxalate crystal under microaerobic conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9689598/
https://www.ncbi.nlm.nih.gov/pubmed/36359494
http://dx.doi.org/10.3390/diagnostics12112651
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