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Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity

High serum cholesterol is a risk factor for cardiovascular disease (CVD), leading to many fatalities. Traditional palm wine (Elaeis guineensis) and corn beer are noted as potential sources of probiotic bacteria with cholesterol lowering activity. In this study, we isolated and characterised lactic a...

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Autores principales: Fossi, Bertrand Tatsinkou, Ekabe, Dickson Ebwelle, Toukam, Liliane Laure, Tatsilong Pambou, Henri Olivier, Gagneux-Brunon, Amandine, Nkenfou Nguefeu, Céline, Bongue, Bienvenu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691922/
https://www.ncbi.nlm.nih.gov/pubmed/36439729
http://dx.doi.org/10.1016/j.heliyon.2022.e11708
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author Fossi, Bertrand Tatsinkou
Ekabe, Dickson Ebwelle
Toukam, Liliane Laure
Tatsilong Pambou, Henri Olivier
Gagneux-Brunon, Amandine
Nkenfou Nguefeu, Céline
Bongue, Bienvenu
author_facet Fossi, Bertrand Tatsinkou
Ekabe, Dickson Ebwelle
Toukam, Liliane Laure
Tatsilong Pambou, Henri Olivier
Gagneux-Brunon, Amandine
Nkenfou Nguefeu, Céline
Bongue, Bienvenu
author_sort Fossi, Bertrand Tatsinkou
collection PubMed
description High serum cholesterol is a risk factor for cardiovascular disease (CVD), leading to many fatalities. Traditional palm wine (Elaeis guineensis) and corn beer are noted as potential sources of probiotic bacteria with cholesterol lowering activity. In this study, we isolated and characterised lactic acid bacteria (LAB) with probiotic and cholesterol lowering properties from these sources. The isolates were identified using API 50 CHL kit and sequencing of 16 S rRNA gene. A hypercholesterolemia model involving three groups of eighteen diet-induced Wistar albino rats was established, and the serum lipid profile was analysed using biochemical kits. The genotypic identification of two best cholesterol lowering LAB revealed that they were strains of Levilactobacillus brevis and Enterococcus faeccium. Their 16 S r RNA gene sequences were deposited in NCBI genbank with the accension numbers ON454506 and ON908682 respectively. These isolates effectively lowered LDL-c and increased HDL-c in rat sera, which are the main risk factors for CVD. These probiotics are potential candidates for functional foods formulation against hypercholesterolemia.
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spelling pubmed-96919222022-11-26 Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity Fossi, Bertrand Tatsinkou Ekabe, Dickson Ebwelle Toukam, Liliane Laure Tatsilong Pambou, Henri Olivier Gagneux-Brunon, Amandine Nkenfou Nguefeu, Céline Bongue, Bienvenu Heliyon Research Article High serum cholesterol is a risk factor for cardiovascular disease (CVD), leading to many fatalities. Traditional palm wine (Elaeis guineensis) and corn beer are noted as potential sources of probiotic bacteria with cholesterol lowering activity. In this study, we isolated and characterised lactic acid bacteria (LAB) with probiotic and cholesterol lowering properties from these sources. The isolates were identified using API 50 CHL kit and sequencing of 16 S rRNA gene. A hypercholesterolemia model involving three groups of eighteen diet-induced Wistar albino rats was established, and the serum lipid profile was analysed using biochemical kits. The genotypic identification of two best cholesterol lowering LAB revealed that they were strains of Levilactobacillus brevis and Enterococcus faeccium. Their 16 S r RNA gene sequences were deposited in NCBI genbank with the accension numbers ON454506 and ON908682 respectively. These isolates effectively lowered LDL-c and increased HDL-c in rat sera, which are the main risk factors for CVD. These probiotics are potential candidates for functional foods formulation against hypercholesterolemia. Elsevier 2022-11-17 /pmc/articles/PMC9691922/ /pubmed/36439729 http://dx.doi.org/10.1016/j.heliyon.2022.e11708 Text en © 2022 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Fossi, Bertrand Tatsinkou
Ekabe, Dickson Ebwelle
Toukam, Liliane Laure
Tatsilong Pambou, Henri Olivier
Gagneux-Brunon, Amandine
Nkenfou Nguefeu, Céline
Bongue, Bienvenu
Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity
title Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity
title_full Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity
title_fullStr Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity
title_full_unstemmed Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity
title_short Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity
title_sort probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691922/
https://www.ncbi.nlm.nih.gov/pubmed/36439729
http://dx.doi.org/10.1016/j.heliyon.2022.e11708
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