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Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity
High serum cholesterol is a risk factor for cardiovascular disease (CVD), leading to many fatalities. Traditional palm wine (Elaeis guineensis) and corn beer are noted as potential sources of probiotic bacteria with cholesterol lowering activity. In this study, we isolated and characterised lactic a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691922/ https://www.ncbi.nlm.nih.gov/pubmed/36439729 http://dx.doi.org/10.1016/j.heliyon.2022.e11708 |
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author | Fossi, Bertrand Tatsinkou Ekabe, Dickson Ebwelle Toukam, Liliane Laure Tatsilong Pambou, Henri Olivier Gagneux-Brunon, Amandine Nkenfou Nguefeu, Céline Bongue, Bienvenu |
author_facet | Fossi, Bertrand Tatsinkou Ekabe, Dickson Ebwelle Toukam, Liliane Laure Tatsilong Pambou, Henri Olivier Gagneux-Brunon, Amandine Nkenfou Nguefeu, Céline Bongue, Bienvenu |
author_sort | Fossi, Bertrand Tatsinkou |
collection | PubMed |
description | High serum cholesterol is a risk factor for cardiovascular disease (CVD), leading to many fatalities. Traditional palm wine (Elaeis guineensis) and corn beer are noted as potential sources of probiotic bacteria with cholesterol lowering activity. In this study, we isolated and characterised lactic acid bacteria (LAB) with probiotic and cholesterol lowering properties from these sources. The isolates were identified using API 50 CHL kit and sequencing of 16 S rRNA gene. A hypercholesterolemia model involving three groups of eighteen diet-induced Wistar albino rats was established, and the serum lipid profile was analysed using biochemical kits. The genotypic identification of two best cholesterol lowering LAB revealed that they were strains of Levilactobacillus brevis and Enterococcus faeccium. Their 16 S r RNA gene sequences were deposited in NCBI genbank with the accension numbers ON454506 and ON908682 respectively. These isolates effectively lowered LDL-c and increased HDL-c in rat sera, which are the main risk factors for CVD. These probiotics are potential candidates for functional foods formulation against hypercholesterolemia. |
format | Online Article Text |
id | pubmed-9691922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96919222022-11-26 Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity Fossi, Bertrand Tatsinkou Ekabe, Dickson Ebwelle Toukam, Liliane Laure Tatsilong Pambou, Henri Olivier Gagneux-Brunon, Amandine Nkenfou Nguefeu, Céline Bongue, Bienvenu Heliyon Research Article High serum cholesterol is a risk factor for cardiovascular disease (CVD), leading to many fatalities. Traditional palm wine (Elaeis guineensis) and corn beer are noted as potential sources of probiotic bacteria with cholesterol lowering activity. In this study, we isolated and characterised lactic acid bacteria (LAB) with probiotic and cholesterol lowering properties from these sources. The isolates were identified using API 50 CHL kit and sequencing of 16 S rRNA gene. A hypercholesterolemia model involving three groups of eighteen diet-induced Wistar albino rats was established, and the serum lipid profile was analysed using biochemical kits. The genotypic identification of two best cholesterol lowering LAB revealed that they were strains of Levilactobacillus brevis and Enterococcus faeccium. Their 16 S r RNA gene sequences were deposited in NCBI genbank with the accension numbers ON454506 and ON908682 respectively. These isolates effectively lowered LDL-c and increased HDL-c in rat sera, which are the main risk factors for CVD. These probiotics are potential candidates for functional foods formulation against hypercholesterolemia. Elsevier 2022-11-17 /pmc/articles/PMC9691922/ /pubmed/36439729 http://dx.doi.org/10.1016/j.heliyon.2022.e11708 Text en © 2022 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Fossi, Bertrand Tatsinkou Ekabe, Dickson Ebwelle Toukam, Liliane Laure Tatsilong Pambou, Henri Olivier Gagneux-Brunon, Amandine Nkenfou Nguefeu, Céline Bongue, Bienvenu Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity |
title | Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity |
title_full | Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity |
title_fullStr | Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity |
title_full_unstemmed | Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity |
title_short | Probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity |
title_sort | probiotic lactic acid bacteria isolated from traditional cameroonian palm wine and corn beer exhibiting cholesterol lowering activity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691922/ https://www.ncbi.nlm.nih.gov/pubmed/36439729 http://dx.doi.org/10.1016/j.heliyon.2022.e11708 |
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