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Protocol to analyze lipid asymmetry in the plasma membrane

The plasma membrane containing cholesterol exhibits phospholipid asymmetry, with phosphatidylcholine and sphingomyelin enriched in its outer leaflet and phosphatidylserine (PtdSer) and phosphatidylethanolamine (PtdEtn) on the cytoplasmic side. We herein describe steps for bacterial expression of rec...

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Detalles Bibliográficos
Autores principales: Miyata, Yugo, Segawa, Katsumori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692065/
https://www.ncbi.nlm.nih.gov/pubmed/36595929
http://dx.doi.org/10.1016/j.xpro.2022.101870