Cargando…

Protocol to analyze lipid asymmetry in the plasma membrane

The plasma membrane containing cholesterol exhibits phospholipid asymmetry, with phosphatidylcholine and sphingomyelin enriched in its outer leaflet and phosphatidylserine (PtdSer) and phosphatidylethanolamine (PtdEtn) on the cytoplasmic side. We herein describe steps for bacterial expression of rec...

Descripción completa

Detalles Bibliográficos
Autores principales:	Miyata, Yugo, Segawa, Katsumori
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692065/ https://www.ncbi.nlm.nih.gov/pubmed/36595929 http://dx.doi.org/10.1016/j.xpro.2022.101870

Ejemplares similares

Two types of type IV P-type ATPases independently re-establish the asymmetrical distribution of phosphatidylserine in plasma membranes
por: Miyata, Yugo, et al.
Publicado: (2022)

Model Membrane Systems Used to Study Plasma Membrane Lipid Asymmetry
por: Scott, Haden L., et al.
Publicado: (2021)

Preparation of Artificial Plasma Membrane Mimicking Vesicles with Lipid Asymmetry
por: Lin, Qingqing, et al.
Publicado: (2014)

Protocol for electron microscopy ultrastructural localization of the fusogenic lipid phosphatidic acid on plasma membrane sheets from chromaffin cells
por: Tanguy, Emeline, et al.
Publicado: (2021)

An optimized protocol to analyze membrane protein degradation in yeast using quantitative western blot and flow cytometry
por: Arines, Felichi Mae, et al.
Publicado: (2022)

Protocol for evaluating protein relocalization from the plasma membrane to chloroplasts
por: Medina-Puche, Laura, et al.
Publicado: (2021)

DANAMIC: Data analyzer of minimum inhibitory concentrations – Protocol to analyze antimicrobial susceptibility data
por: Garcia-Ruiz, Javier, et al.
Publicado: (2022)

Labeling and analyzing lipid droplets in mouse muscle stem cells
por: Chen, Jingjuan, et al.
Publicado: (2022)

Protocol for recording and analyzing spontaneous locomotion in Drosophila
por: Barwell, Taylor, et al.
Publicado: (2022)

Protocol to analyze circulating small non-coding RNAs by high-throughput RNA sequencing from human plasma samples
por: Grieco, Giuseppina E., et al.
Publicado: (2021)

Comment on ‘Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol’
por: Courtney, Kevin C, et al.
Publicado: (2018)

Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining
por: Baum, Julia F., et al.
Publicado: (2023)

Superelasticity of Plasma‐ and Synthetic Membranes Resulting from Coupling of Membrane Asymmetry, Curvature, and Lipid Sorting
por: Steinkühler, Jan, et al.
Publicado: (2021)

Loss of plasma membrane lipid asymmetry can induce ordered domain (raft) formation
por: Kakuda, Shinako, et al.
Publicado: (2021)

A protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles
por: Zhu, Yuanxiang, et al.
Publicado: (2022)

Requirement of Xk and Vps13a for the P2X7-mediated phospholipid scrambling and cell lysis in mouse T cells
por: Ryoden, Yuta, et al.
Publicado: (2022)

Electron microscopy combined with spatial analysis: quantitative mapping of the nano-assemblies of plasma membrane-associating proteins and lipids
por: Zhou, Yong, et al.
Publicado: (2018)

Protocol to analyze the bacterial pangenome using PAN2HGENE software
por: de Sá, Pablo Henrique Caracciolo Gomes, et al.
Publicado: (2022)

Protocol for designing, optimizing, and analyzing reverse supply chains using RELOG
por: Iloeje, Chukwunwike O., et al.
Publicado: (2022)

Protocol for analyzing the biosynthesis and degradation of N-glycan precursors in mammalian cells
por: Harada, Yoichiro, et al.
Publicado: (2021)

Protocol for monitoring and analyzing single nucleotide incorporation by S. cerevisiae RNA polymerases
por: Jacobs, Ruth Q., et al.
Publicado: (2023)

Protocol to analyze endogenous translesion DNA synthesis in single mammalian cells
por: Egger, Tom, et al.
Publicado: (2023)

The lepton asymmetry analyzer: LAA
por: CERN. Geneva. SPS Experiments Committee
Publicado: (1986)

An adaptable live-cell imaging protocol to analyze organelle morphology in Saccharomyces cerevisiae
por: Deolal, Pallavi, et al.
Publicado: (2022)

Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia
por: Erny, Daniel, et al.
Publicado: (2022)

Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)
por: Sauter, Manuela, et al.
Publicado: (2022)

Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry
por: Zhang, Yuwei, et al.
Publicado: (2020)

Protocol for analyzing enzymatic hydrolysis of cellulose using surface pitting observation technology
por: Tachioka, Mikako, et al.
Publicado: (2023)

Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana
por: Yu, Bo, et al.
Publicado: (2023)

Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro
por: Schoppmeyer, Rouven, et al.
Publicado: (2022)

Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy
por: Nakai-Shimoda, Hiromi, et al.
Publicado: (2022)

Protocol to analyze population structure and migration history based on human genome variation data
por: Zhao, Zicheng, et al.
Publicado: (2022)

A protocol for analyzing the protein terminome of human cancer cell line culture supernatants
por: Tsumagari, Kazuya, et al.
Publicado: (2021)

Protocols for analyzing metabolic derangements caused by increased NADH/NAD(+) ratio in cell lines and in mice
por: Liu, Shanshan, et al.
Publicado: (2022)

Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo
por: Ren, Deshan, et al.
Publicado: (2022)

Protocol to analyze the transmigration efficiency of T. brucei using an in vitro model of the blood-cerebrospinal fluid barrier
por: Herrmann, Alexander, et al.
Publicado: (2022)

Protocol to dissociate, process, and analyze the human lung tissue using single-cell RNA-seq
por: Quintanal-Villalonga, Álvaro, et al.
Publicado: (2022)

Protocol for analyzing protein ensemble structures from chemical cross-links using DynaXL
por: Gong, Zhou, et al.
Publicado: (2017)

A Systematic Framework for Analyzing Patient-Generated Narrative Data: Protocol for a Content Analysis
por: Zolnoori, Maryam, et al.
Publicado: (2019)

Protocol for analyzing and visualizing antiviral immune responses after acute infection of the murine oral mucosa
por: Shannon, John P., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_9dk6slaf2fl792clbj52bulsn2