Protocol to use de-epithelialized porcine urinary bladder as a tissue scaffold for propagation of pancreatic cells

Ex vivo organ culture can be a useful alternative to in vivo models, which can be time-, labor-, and cost-intensive. Here we describe a step-by-step protocol to use de-epithelialized porcine urinary bladders as scaffolds in air-liquid interface in vitro culture systems for a variety of pluripotent s...

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Detalles Bibliográficos
Autores principales: Melzer, Michael Karl, Breunig, Markus, Lopatta, Paul, Hohwieler, Meike, Merz, Sarah, Azoitei, Anca, Günes, Cagatay, Bolenz, Christian, Kleger, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692066/
https://www.ncbi.nlm.nih.gov/pubmed/36595896
http://dx.doi.org/10.1016/j.xpro.2022.101869

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692066/
https://www.ncbi.nlm.nih.gov/pubmed/36595896
http://dx.doi.org/10.1016/j.xpro.2022.101869

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