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Protocol to use de-epithelialized porcine urinary bladder as a tissue scaffold for propagation of pancreatic cells
Ex vivo organ culture can be a useful alternative to in vivo models, which can be time-, labor-, and cost-intensive. Here we describe a step-by-step protocol to use de-epithelialized porcine urinary bladders as scaffolds in air-liquid interface in vitro culture systems for a variety of pluripotent s...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692066/ https://www.ncbi.nlm.nih.gov/pubmed/36595896 http://dx.doi.org/10.1016/j.xpro.2022.101869 |