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Creation of a new Drosophila melanogaster X chromosome balancer, First Multiple 8 ( FM8 ), using the CRISPR/Cas9 gene-editing system

Balancer chromosomes contain multiple inversions that work to suppress crossing over and prevent recovery of most recombinant chromosomes, allowing for alleles to be kept long-term without selection. These balancers are incredible tools, but some alleles within larger inverted segments are still los...

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Detalles Bibliográficos
Autores principales: Muron, Savannah, Kucera, Steve, Oliver, Brian, Benner, Leif
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692231/
https://www.ncbi.nlm.nih.gov/pubmed/36439394
http://dx.doi.org/10.17912/micropub.biology.000663
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author Muron, Savannah
Kucera, Steve
Oliver, Brian
Benner, Leif
author_facet Muron, Savannah
Kucera, Steve
Oliver, Brian
Benner, Leif
author_sort Muron, Savannah
collection PubMed
description Balancer chromosomes contain multiple inversions that work to suppress crossing over and prevent recovery of most recombinant chromosomes, allowing for alleles to be kept long-term without selection. These balancers are incredible tools, but some alleles within larger inverted segments are still lost to rare double crossover events between the balanced and balancer chromosomes. This study details a new methodology of producing balancer chromosomes using CRISPR/Cas9 gene editing technology to create new inversions within the largest segment of the common X chromosome balancer, FM7c. We were able to create a new X chromosome balancer, FM8, and anticipate that this process can be used to not only create other balancers in Drosophila melanogaster, but other model organisms as well.
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spelling pubmed-96922312022-11-26 Creation of a new Drosophila melanogaster X chromosome balancer, First Multiple 8 ( FM8 ), using the CRISPR/Cas9 gene-editing system Muron, Savannah Kucera, Steve Oliver, Brian Benner, Leif MicroPubl Biol New Finding Balancer chromosomes contain multiple inversions that work to suppress crossing over and prevent recovery of most recombinant chromosomes, allowing for alleles to be kept long-term without selection. These balancers are incredible tools, but some alleles within larger inverted segments are still lost to rare double crossover events between the balanced and balancer chromosomes. This study details a new methodology of producing balancer chromosomes using CRISPR/Cas9 gene editing technology to create new inversions within the largest segment of the common X chromosome balancer, FM7c. We were able to create a new X chromosome balancer, FM8, and anticipate that this process can be used to not only create other balancers in Drosophila melanogaster, but other model organisms as well. Caltech Library 2022-11-10 /pmc/articles/PMC9692231/ /pubmed/36439394 http://dx.doi.org/10.17912/micropub.biology.000663 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Muron, Savannah
Kucera, Steve
Oliver, Brian
Benner, Leif
Creation of a new Drosophila melanogaster X chromosome balancer, First Multiple 8 ( FM8 ), using the CRISPR/Cas9 gene-editing system
title Creation of a new Drosophila melanogaster X chromosome balancer, First Multiple 8 ( FM8 ), using the CRISPR/Cas9 gene-editing system
title_full Creation of a new Drosophila melanogaster X chromosome balancer, First Multiple 8 ( FM8 ), using the CRISPR/Cas9 gene-editing system
title_fullStr Creation of a new Drosophila melanogaster X chromosome balancer, First Multiple 8 ( FM8 ), using the CRISPR/Cas9 gene-editing system
title_full_unstemmed Creation of a new Drosophila melanogaster X chromosome balancer, First Multiple 8 ( FM8 ), using the CRISPR/Cas9 gene-editing system
title_short Creation of a new Drosophila melanogaster X chromosome balancer, First Multiple 8 ( FM8 ), using the CRISPR/Cas9 gene-editing system
title_sort creation of a new drosophila melanogaster x chromosome balancer, first multiple 8 ( fm8 ), using the crispr/cas9 gene-editing system
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692231/
https://www.ncbi.nlm.nih.gov/pubmed/36439394
http://dx.doi.org/10.17912/micropub.biology.000663
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