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Bioluminescence Production by Turnip Yellows Virus Infectious Clones: A New Way to Monitor Plant Virus Infection

We used the NanoLuc luciferase bioluminescent reporter system to detect turnip yellows virus (TuYV) in infected plants. For this, TuYV was genetically tagged by replacing the C-terminal part of the RT protein with full-length NanoLuc (TuYV-NL) or with the N-terminal domain of split NanoLuc (TuYV-N65...

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Detalles Bibliográficos
Autores principales: Boissinot, Sylvaine, Ducousso, Marie, Brault, Véronique, Drucker, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692398/
https://www.ncbi.nlm.nih.gov/pubmed/36430165
http://dx.doi.org/10.3390/ijms232213685