Development of a Molecular Aptamer Beacon Applied to Magnetic-Assisted RNA Extraction for Detection of Dengue and Zika Viruses Using Clinical Samples

Limitations in the detection of cocirculating flaviviruses such as Dengue and Zika lead us to propose the use of aptameric capture of the viral RNA in combination with RT-PCR (APTA-RT-PCR). Aptamers were obtained via SELEX and next-generation sequencing, followed by colorimetric and fluorescent char...

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Detalles Bibliográficos
Autores principales: da Silva, Amanda Gabrielle, Goulart, Luiz Ricardo, Löffler, Philipp, Code, Christian, Neves, Adriana Freitas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693377/
https://www.ncbi.nlm.nih.gov/pubmed/36430340
http://dx.doi.org/10.3390/ijms232213866

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693377/
https://www.ncbi.nlm.nih.gov/pubmed/36430340
http://dx.doi.org/10.3390/ijms232213866

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