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Distribution and Characterization of Antimicrobial Resistant Pathogens in a Pig Farm, Slaughterhouse, Meat Processing Plant, and in Retail Stores

The emergence of antibiotic resistance in foodborne pathogens isolated from meat pro-ducts and their producing environment has been an increasing and leading threat to public health. The aim of the study was to identify pathogens and their antimicrobial resistance isolated from pig production to por...

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Autores principales: Bae, Dongryeoul, Macoy, Donah Mary, Ahmad, Waqas, Peseth, Son, Kim, Binn, Chon, Jung-Whan, Ryu, Gyeong Ryul, Ban, Ga-Hee, Kim, Sun Ae, Kang, Hye Jeong, Moon, Jin San, Kim, Min Gab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693440/
https://www.ncbi.nlm.nih.gov/pubmed/36422322
http://dx.doi.org/10.3390/microorganisms10112252
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author Bae, Dongryeoul
Macoy, Donah Mary
Ahmad, Waqas
Peseth, Son
Kim, Binn
Chon, Jung-Whan
Ryu, Gyeong Ryul
Ban, Ga-Hee
Kim, Sun Ae
Kang, Hye Jeong
Moon, Jin San
Kim, Min Gab
author_facet Bae, Dongryeoul
Macoy, Donah Mary
Ahmad, Waqas
Peseth, Son
Kim, Binn
Chon, Jung-Whan
Ryu, Gyeong Ryul
Ban, Ga-Hee
Kim, Sun Ae
Kang, Hye Jeong
Moon, Jin San
Kim, Min Gab
author_sort Bae, Dongryeoul
collection PubMed
description The emergence of antibiotic resistance in foodborne pathogens isolated from meat pro-ducts and their producing environment has been an increasing and leading threat to public health. The aim of the study was to identify pathogens and their antimicrobial resistance isolated from pig production to pork meat distribution phases. Through this study, food spoilage and foodborne or clinical pathogenic bacteria were isolated and identified from pork (belly and neck) meat product and its related environmental samples that include pig swabs, diets, feces, liquid manure, workers’ gloves, dust fan swabs, carcass swabs, floor swabs, and drain water in the affiliated farm, slaughterhouse, meat processing plant, and in retail stores. All carcasses at the slaughterhouse and meat products at the meat processing plant were tracked from pigs at a targeted farm. Nine different selective media agars were used to effectively isolate various pathogenic bacteria. A total of 283 presumptive pathogenic bacteria isolated from 126 samples were selected and identified using MALDI-ToF MS. Twenty-three important foodborne pathogens were identified, and some of them, Shiga-toxin-producing E. coli (STEC), Listeria monocytogenes, Staphylococcus aureus, and Yersinia enterocolitica, were further confirmed using PCR. The PFGE patterns of 12 STEC isolates were grouped by sample source or site. All the foodborne pathogens used in the study were not resistant to amoxicillin/clavulanate, ciprofloxacin, and gentamicin, whereas some of the STEC, L. monocytogenes, and S. aureus isolates were resistant to various antibiotics, including ampicillin, erythromycin, tetracycline, and vancomycin. The most common antimicrobial resistance pattern in the pathogenic STEC isolates was AMP-KAN-STR-SXT-TET. Consequently, this study provides valuable information for the distribution of antimicrobial-resistant pathogens along the pork meat production chain and can assist farmers and stakeholders to develop a systematic strategy for reducing the current emergence and spread of antimicrobial resistance in the different phases of pig production and distribution.
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spelling pubmed-96934402022-11-26 Distribution and Characterization of Antimicrobial Resistant Pathogens in a Pig Farm, Slaughterhouse, Meat Processing Plant, and in Retail Stores Bae, Dongryeoul Macoy, Donah Mary Ahmad, Waqas Peseth, Son Kim, Binn Chon, Jung-Whan Ryu, Gyeong Ryul Ban, Ga-Hee Kim, Sun Ae Kang, Hye Jeong Moon, Jin San Kim, Min Gab Microorganisms Article The emergence of antibiotic resistance in foodborne pathogens isolated from meat pro-ducts and their producing environment has been an increasing and leading threat to public health. The aim of the study was to identify pathogens and their antimicrobial resistance isolated from pig production to pork meat distribution phases. Through this study, food spoilage and foodborne or clinical pathogenic bacteria were isolated and identified from pork (belly and neck) meat product and its related environmental samples that include pig swabs, diets, feces, liquid manure, workers’ gloves, dust fan swabs, carcass swabs, floor swabs, and drain water in the affiliated farm, slaughterhouse, meat processing plant, and in retail stores. All carcasses at the slaughterhouse and meat products at the meat processing plant were tracked from pigs at a targeted farm. Nine different selective media agars were used to effectively isolate various pathogenic bacteria. A total of 283 presumptive pathogenic bacteria isolated from 126 samples were selected and identified using MALDI-ToF MS. Twenty-three important foodborne pathogens were identified, and some of them, Shiga-toxin-producing E. coli (STEC), Listeria monocytogenes, Staphylococcus aureus, and Yersinia enterocolitica, were further confirmed using PCR. The PFGE patterns of 12 STEC isolates were grouped by sample source or site. All the foodborne pathogens used in the study were not resistant to amoxicillin/clavulanate, ciprofloxacin, and gentamicin, whereas some of the STEC, L. monocytogenes, and S. aureus isolates were resistant to various antibiotics, including ampicillin, erythromycin, tetracycline, and vancomycin. The most common antimicrobial resistance pattern in the pathogenic STEC isolates was AMP-KAN-STR-SXT-TET. Consequently, this study provides valuable information for the distribution of antimicrobial-resistant pathogens along the pork meat production chain and can assist farmers and stakeholders to develop a systematic strategy for reducing the current emergence and spread of antimicrobial resistance in the different phases of pig production and distribution. MDPI 2022-11-14 /pmc/articles/PMC9693440/ /pubmed/36422322 http://dx.doi.org/10.3390/microorganisms10112252 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bae, Dongryeoul
Macoy, Donah Mary
Ahmad, Waqas
Peseth, Son
Kim, Binn
Chon, Jung-Whan
Ryu, Gyeong Ryul
Ban, Ga-Hee
Kim, Sun Ae
Kang, Hye Jeong
Moon, Jin San
Kim, Min Gab
Distribution and Characterization of Antimicrobial Resistant Pathogens in a Pig Farm, Slaughterhouse, Meat Processing Plant, and in Retail Stores
title Distribution and Characterization of Antimicrobial Resistant Pathogens in a Pig Farm, Slaughterhouse, Meat Processing Plant, and in Retail Stores
title_full Distribution and Characterization of Antimicrobial Resistant Pathogens in a Pig Farm, Slaughterhouse, Meat Processing Plant, and in Retail Stores
title_fullStr Distribution and Characterization of Antimicrobial Resistant Pathogens in a Pig Farm, Slaughterhouse, Meat Processing Plant, and in Retail Stores
title_full_unstemmed Distribution and Characterization of Antimicrobial Resistant Pathogens in a Pig Farm, Slaughterhouse, Meat Processing Plant, and in Retail Stores
title_short Distribution and Characterization of Antimicrobial Resistant Pathogens in a Pig Farm, Slaughterhouse, Meat Processing Plant, and in Retail Stores
title_sort distribution and characterization of antimicrobial resistant pathogens in a pig farm, slaughterhouse, meat processing plant, and in retail stores
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693440/
https://www.ncbi.nlm.nih.gov/pubmed/36422322
http://dx.doi.org/10.3390/microorganisms10112252
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