Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles
The research subject of this paper are natural polymer-based hydrogels modified with albumin particles. The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy. The most favorable co...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694906/ https://www.ncbi.nlm.nih.gov/pubmed/36430612 http://dx.doi.org/10.3390/ijms232214136 |
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author | Bańkosz, Magdalena |
author_facet | Bańkosz, Magdalena |
author_sort | Bańkosz, Magdalena |
collection | PubMed |
description | The research subject of this paper are natural polymer-based hydrogels modified with albumin particles. The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy. The most favorable composition showing monodispersity and particles with a size lower than 40 nm was selected for modification of hydrogels. Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator. Next, the hydrogels’ swelling ability, mechanical properties, wettability and surface morphology were characterized. Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed. The release profiles of proteins from hydrogels were also verified. Materials modified with proteins showed higher swelling ability, increased flexibility even by 50% and increased surface hydrophilicity. Hydrogels’ contact angles were within the range 62–69° while the tensile strength of albumin-containing hydrogels was approx. 0.11 MPa. Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined. Incubation studies showed hydrogels’ stability and lack of their degradation in tested media. The viability of fibroblasts was 89.54% for unmodified hydrogel, and approx. 92.73% for albumin-modified hydrogel, and such an increase indicated the positive impact of the albumin on murine fibroblast proliferation. |
format | Online Article Text |
id | pubmed-9694906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96949062022-11-26 Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles Bańkosz, Magdalena Int J Mol Sci Article The research subject of this paper are natural polymer-based hydrogels modified with albumin particles. The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy. The most favorable composition showing monodispersity and particles with a size lower than 40 nm was selected for modification of hydrogels. Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator. Next, the hydrogels’ swelling ability, mechanical properties, wettability and surface morphology were characterized. Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed. The release profiles of proteins from hydrogels were also verified. Materials modified with proteins showed higher swelling ability, increased flexibility even by 50% and increased surface hydrophilicity. Hydrogels’ contact angles were within the range 62–69° while the tensile strength of albumin-containing hydrogels was approx. 0.11 MPa. Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined. Incubation studies showed hydrogels’ stability and lack of their degradation in tested media. The viability of fibroblasts was 89.54% for unmodified hydrogel, and approx. 92.73% for albumin-modified hydrogel, and such an increase indicated the positive impact of the albumin on murine fibroblast proliferation. MDPI 2022-11-16 /pmc/articles/PMC9694906/ /pubmed/36430612 http://dx.doi.org/10.3390/ijms232214136 Text en © 2022 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bańkosz, Magdalena Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles |
title | Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles |
title_full | Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles |
title_fullStr | Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles |
title_full_unstemmed | Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles |
title_short | Development of Chitosan/Gelatin-Based Hydrogels Incorporated with Albumin Particles |
title_sort | development of chitosan/gelatin-based hydrogels incorporated with albumin particles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694906/ https://www.ncbi.nlm.nih.gov/pubmed/36430612 http://dx.doi.org/10.3390/ijms232214136 |
work_keys_str_mv | AT bankoszmagdalena developmentofchitosangelatinbasedhydrogelsincorporatedwithalbuminparticles |