Recombinant Globular Domain of TcpA Pilin from Vibrio cholerae El Tor: Recovery from Inclusion Bodies and Structural Characterization

The production of recombinant proteins in Escherichia coli cells is often hampered by aggregation of newly synthesized proteins and formation of inclusion bodies. Here we propose the use of transverse urea gradient electrophoresis (TUGE) in testing the capability of folding of a recombinant protein...

Descripción completa

Detalles Bibliográficos
Autores principales: Marchenkov, Victor, Dubovitskya, Elena, Kotova, Nina, Tuchkov, Igor, Smirnova, Nina, Marchenko, Natalia, Surin, Alexey, Filimonov, Vladimir, Semisotnov, Gennady
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695179/
https://www.ncbi.nlm.nih.gov/pubmed/36362959
http://dx.doi.org/10.3390/life12111802

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695179/
https://www.ncbi.nlm.nih.gov/pubmed/36362959
http://dx.doi.org/10.3390/life12111802

Ejemplares similares