Detergent-Assisted Protein Digestion—On the Way to Avoid the Key Bottleneck of Shotgun Bottom-Up Proteomics

Gel-free bottom-up shotgun proteomics is the principal methodological platform for the state-of-the-art proteome research. This methodology assumes quantitative isolation of the total protein fraction from a complex biological sample, its limited proteolysis with site-specific proteases, analysis of...

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Detalles Bibliográficos
Autores principales: Danko, Katerina, Lukasheva, Elena, Zhukov, Vladimir A., Zgoda, Viktor, Frolov, Andrej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695859/
https://www.ncbi.nlm.nih.gov/pubmed/36430380
http://dx.doi.org/10.3390/ijms232213903

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9695859/
https://www.ncbi.nlm.nih.gov/pubmed/36430380
http://dx.doi.org/10.3390/ijms232213903

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