Immobilization of Lipase B from Candida antarctica in Octyl-Vinyl Sulfone Agarose: Effect of the Enzyme-Support Interactions on Enzyme Activity, Specificity, Structure and Inactivation Pathway

Lipase B from Candida antarctica was immobilized on heterofunctional support octyl agarose activated with vinyl sulfone to prevent enzyme release under drastic conditions. Covalent attachment was established, but the blocking step using hexylamine, ethylenediamine or the amino acids glycine (Gly) an...

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Detalles Bibliográficos
Autores principales: Souza, Priscila M. P., Carballares, Diego, Gonçalves, Luciana R. B., Fernandez-Lafuente, Roberto, Rodrigues, Sueli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697615/
https://www.ncbi.nlm.nih.gov/pubmed/36430745
http://dx.doi.org/10.3390/ijms232214268

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697615/
https://www.ncbi.nlm.nih.gov/pubmed/36430745
http://dx.doi.org/10.3390/ijms232214268

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