Alginate–Chitosan Hydrogel Formulations Sustain Baculovirus Delivery and VEGFA Expression Which Promotes Angiogenesis for Wound Dressing Applications

Hydrogel wound dressings are effective in their ability to provide a wound-healing environment but are limited by their ability to promote later stages of revascularization. Here, a biosafe recombinant baculovirus expressing VEGFA tagged with EGFP is encapsulated in chitosan-coated alginate hydrogel...

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Autores principales: Schaly, Sabrina, Islam, Paromita, Abosalha, Ahmed, Boyajian, Jacqueline L., Shum-Tim, Dominique, Prakash, Satya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698055/
https://www.ncbi.nlm.nih.gov/pubmed/36355553
http://dx.doi.org/10.3390/ph15111382
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author Schaly, Sabrina
Islam, Paromita
Abosalha, Ahmed
Boyajian, Jacqueline L.
Shum-Tim, Dominique
Prakash, Satya
author_facet Schaly, Sabrina
Islam, Paromita
Abosalha, Ahmed
Boyajian, Jacqueline L.
Shum-Tim, Dominique
Prakash, Satya
author_sort Schaly, Sabrina
collection PubMed
description Hydrogel wound dressings are effective in their ability to provide a wound-healing environment but are limited by their ability to promote later stages of revascularization. Here, a biosafe recombinant baculovirus expressing VEGFA tagged with EGFP is encapsulated in chitosan-coated alginate hydrogels using ionic cross-linking. The VEGFA, delivered by the baculovirus, significantly improves cell migration and angiogenesis to assist with the wound-healing process and revascularization. Moreover, the hydrogels have an encapsulation efficiency of 99.9%, no cytotoxicity, antimicrobial properties, good blood compatibility, promote hemostasis, and enable sustained delivery of baculoviruses over eight days. These hydrogels sustain baculovirus delivery and may have clinical implications in wound dressings or future gene therapy applications.
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spelling pubmed-96980552022-11-26 Alginate–Chitosan Hydrogel Formulations Sustain Baculovirus Delivery and VEGFA Expression Which Promotes Angiogenesis for Wound Dressing Applications Schaly, Sabrina Islam, Paromita Abosalha, Ahmed Boyajian, Jacqueline L. Shum-Tim, Dominique Prakash, Satya Pharmaceuticals (Basel) Article Hydrogel wound dressings are effective in their ability to provide a wound-healing environment but are limited by their ability to promote later stages of revascularization. Here, a biosafe recombinant baculovirus expressing VEGFA tagged with EGFP is encapsulated in chitosan-coated alginate hydrogels using ionic cross-linking. The VEGFA, delivered by the baculovirus, significantly improves cell migration and angiogenesis to assist with the wound-healing process and revascularization. Moreover, the hydrogels have an encapsulation efficiency of 99.9%, no cytotoxicity, antimicrobial properties, good blood compatibility, promote hemostasis, and enable sustained delivery of baculoviruses over eight days. These hydrogels sustain baculovirus delivery and may have clinical implications in wound dressings or future gene therapy applications. MDPI 2022-11-10 /pmc/articles/PMC9698055/ /pubmed/36355553 http://dx.doi.org/10.3390/ph15111382 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schaly, Sabrina
Islam, Paromita
Abosalha, Ahmed
Boyajian, Jacqueline L.
Shum-Tim, Dominique
Prakash, Satya
Alginate–Chitosan Hydrogel Formulations Sustain Baculovirus Delivery and VEGFA Expression Which Promotes Angiogenesis for Wound Dressing Applications
title Alginate–Chitosan Hydrogel Formulations Sustain Baculovirus Delivery and VEGFA Expression Which Promotes Angiogenesis for Wound Dressing Applications
title_full Alginate–Chitosan Hydrogel Formulations Sustain Baculovirus Delivery and VEGFA Expression Which Promotes Angiogenesis for Wound Dressing Applications
title_fullStr Alginate–Chitosan Hydrogel Formulations Sustain Baculovirus Delivery and VEGFA Expression Which Promotes Angiogenesis for Wound Dressing Applications
title_full_unstemmed Alginate–Chitosan Hydrogel Formulations Sustain Baculovirus Delivery and VEGFA Expression Which Promotes Angiogenesis for Wound Dressing Applications
title_short Alginate–Chitosan Hydrogel Formulations Sustain Baculovirus Delivery and VEGFA Expression Which Promotes Angiogenesis for Wound Dressing Applications
title_sort alginate–chitosan hydrogel formulations sustain baculovirus delivery and vegfa expression which promotes angiogenesis for wound dressing applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698055/
https://www.ncbi.nlm.nih.gov/pubmed/36355553
http://dx.doi.org/10.3390/ph15111382
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