New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease

The RNA-guided Cas12a nuclease forms a complex with a CRISPR RNA (crRNA) to cleave the double-stranded DNA target. Among others, Cas12a protein from Lachnospiraceae bacterium (LbCas12a) is widely used for biomedical research. For target recognition, LbCas12a requires a specific nucleotide sequence,...

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Detalles Bibliográficos
Autores principales: Misiurina, Mariia A., Chirinskaite, Angelina V., Fotina, Aleksandra S., Zelinsky, Andrey A., Sopova, Julia V., Leonova, Elena I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698171/
https://www.ncbi.nlm.nih.gov/pubmed/36431062
http://dx.doi.org/10.3390/life12111927

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698171/
https://www.ncbi.nlm.nih.gov/pubmed/36431062
http://dx.doi.org/10.3390/life12111927

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