Immunostaining of tumor-infiltrating immune-cell-related markers and cytokines in the tumor fractional radiation model of host PD-L1-deficient mice

In this protocol, we describe how to explore immune function in radiation-induced anti-tumor abscopal effect (ATAE) in PD-L1-deficient (PD-L1(−/−)) mice. We detail steps for primary and abscopal tumor model establishment in mice, tumor fractional irradiation scheme, and isolation of single cells fro...

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Detalles Bibliográficos
Autores principales: Zhao, Xinrui, Shao, Chunlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700008/
https://www.ncbi.nlm.nih.gov/pubmed/36595921
http://dx.doi.org/10.1016/j.xpro.2022.101859

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700008/
https://www.ncbi.nlm.nih.gov/pubmed/36595921
http://dx.doi.org/10.1016/j.xpro.2022.101859

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