Increasing protein stability by inferring substitution effects from high-throughput experiments

We apply a computational model, global multi-mutant analysis (GMMA), to inform on effects of most amino acid substitutions from a randomly mutated gene library. Using a high mutation frequency, the method can determine mutations that increase the stability of even very stable proteins for which conv...

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Detalles Bibliográficos
Autores principales: Norrild, Rasmus Krogh, Johansson, Kristoffer Enøe, O’Shea, Charlotte, Morth, Jens Preben, Lindorff-Larsen, Kresten, Winther, Jakob Rahr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701609/
https://www.ncbi.nlm.nih.gov/pubmed/36452862
http://dx.doi.org/10.1016/j.crmeth.2022.100333

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701609/
https://www.ncbi.nlm.nih.gov/pubmed/36452862
http://dx.doi.org/10.1016/j.crmeth.2022.100333

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