Necessity of integrated genomic analysis to establish a designed knock-in mouse from CRISPR-Cas9-induced mutants

The CRISPR-Cas9 method for generation of knock-in mutations in rodent embryos yields many F0 generation candidates that may have the designed mutations. The first task for selection of promising F0 generations is to analyze genomic DNA which likely contains a mixture of designed and unexpected mutat...

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Detalles Bibliográficos
Autores principales: Yoshida, Masahide, Saito, Tomoko, Takayanagi, Yuki, Totsuka, Yoshikazu, Onaka, Tatsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701781/
https://www.ncbi.nlm.nih.gov/pubmed/36437283
http://dx.doi.org/10.1038/s41598-022-24810-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701781/
https://www.ncbi.nlm.nih.gov/pubmed/36437283
http://dx.doi.org/10.1038/s41598-022-24810-5

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