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Repeated subsamples during DNA extraction reveal increased diversity estimates in DNA metabarcoding of Malaise traps

With increased application of DNA metabarcoding in biodiversity assessment, various laboratory protocols have been optimized, and their further evaluation is subject of current research. Homogenization of bulk samples and subsequent DNA extraction from a subsample of destructed tissue is a common fi...

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Detalles Bibliográficos
Autores principales: Zizka, Vera M. A., Geiger, Matthias F., Hörren, Thomas, Kirse, Ameli, Noll, Niklas W., Schäffler, Livia, Scherges, Alice M., Sorg, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9702565/
https://www.ncbi.nlm.nih.gov/pubmed/36447594
http://dx.doi.org/10.1002/ece3.9502