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Identification and characterization of circRNAs related to meat quality during embryonic development of the longissimus dorsi muscle in two pig breeds

Meat quality, an important economic trait, is regulated by many factors, especially by genetic factors, including coding genes, miRNAs, and lncRNAs. Recent studies have elucidated that circRNAs also play a key role in muscle development and lipid deposition. However, the functions and regulatory mec...

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Autores principales: Wang, Jing, Chen, Jun-Feng, Ma, Qiang, Mo, De-Lin, Sun, Jia-Jie, Ren, Qiao-Ling, Zhang, Jia-Qing, Lu, Qing-Xia, Xing, Bao-Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705349/
https://www.ncbi.nlm.nih.gov/pubmed/36457752
http://dx.doi.org/10.3389/fgene.2022.1019687
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author Wang, Jing
Chen, Jun-Feng
Ma, Qiang
Mo, De-Lin
Sun, Jia-Jie
Ren, Qiao-Ling
Zhang, Jia-Qing
Lu, Qing-Xia
Xing, Bao-Song
author_facet Wang, Jing
Chen, Jun-Feng
Ma, Qiang
Mo, De-Lin
Sun, Jia-Jie
Ren, Qiao-Ling
Zhang, Jia-Qing
Lu, Qing-Xia
Xing, Bao-Song
author_sort Wang, Jing
collection PubMed
description Meat quality, an important economic trait, is regulated by many factors, especially by genetic factors, including coding genes, miRNAs, and lncRNAs. Recent studies have elucidated that circRNAs also play a key role in muscle development and lipid deposition. However, the functions and regulatory mechanisms of circRNAs in meat quality remain mostly unknown. The circRNA expression profiles between Huainan pigs (Chinese indigenous pigs, fat-type, Huainan HN) and Large White pigs (Western commercial pigs, lean-type, LW) in the longissimus dorsi (LD) muscle at 38, 58, and 78 days post conception (dpc) were compared by sequencing. In total, 39,887 circRNAs were identified in 18 samples, and 60, 78, and 86 differentially expressed circRNAs (DECs) were found at the three stages mentioned above between these two breeds. The parent genes of DECs were enriched in myogenesis, proliferation, adipogenesis and muscle fiber-type transition. The circRNA-miRNA interaction networks included 38 DECs and 47 miRNAs, and these miRNAs were involved in muscle development and lipid metabolism. Two shared DECs (circ_0030593 and circ_0032760) of these three stages were selected, their head-to-tail junction sites were validated by Sanger sequencing, and RT‒qPCR results suggested that these two DECs might be involved in intramuscular fat deposition. These findings provide a basis for understanding the role of circRNAs in meat quality.
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spelling pubmed-97053492022-11-30 Identification and characterization of circRNAs related to meat quality during embryonic development of the longissimus dorsi muscle in two pig breeds Wang, Jing Chen, Jun-Feng Ma, Qiang Mo, De-Lin Sun, Jia-Jie Ren, Qiao-Ling Zhang, Jia-Qing Lu, Qing-Xia Xing, Bao-Song Front Genet Genetics Meat quality, an important economic trait, is regulated by many factors, especially by genetic factors, including coding genes, miRNAs, and lncRNAs. Recent studies have elucidated that circRNAs also play a key role in muscle development and lipid deposition. However, the functions and regulatory mechanisms of circRNAs in meat quality remain mostly unknown. The circRNA expression profiles between Huainan pigs (Chinese indigenous pigs, fat-type, Huainan HN) and Large White pigs (Western commercial pigs, lean-type, LW) in the longissimus dorsi (LD) muscle at 38, 58, and 78 days post conception (dpc) were compared by sequencing. In total, 39,887 circRNAs were identified in 18 samples, and 60, 78, and 86 differentially expressed circRNAs (DECs) were found at the three stages mentioned above between these two breeds. The parent genes of DECs were enriched in myogenesis, proliferation, adipogenesis and muscle fiber-type transition. The circRNA-miRNA interaction networks included 38 DECs and 47 miRNAs, and these miRNAs were involved in muscle development and lipid metabolism. Two shared DECs (circ_0030593 and circ_0032760) of these three stages were selected, their head-to-tail junction sites were validated by Sanger sequencing, and RT‒qPCR results suggested that these two DECs might be involved in intramuscular fat deposition. These findings provide a basis for understanding the role of circRNAs in meat quality. Frontiers Media S.A. 2022-11-15 /pmc/articles/PMC9705349/ /pubmed/36457752 http://dx.doi.org/10.3389/fgene.2022.1019687 Text en Copyright © 2022 Wang, Chen, Ma, Mo, Sun, Ren, Zhang, Lu and Xing. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Wang, Jing
Chen, Jun-Feng
Ma, Qiang
Mo, De-Lin
Sun, Jia-Jie
Ren, Qiao-Ling
Zhang, Jia-Qing
Lu, Qing-Xia
Xing, Bao-Song
Identification and characterization of circRNAs related to meat quality during embryonic development of the longissimus dorsi muscle in two pig breeds
title Identification and characterization of circRNAs related to meat quality during embryonic development of the longissimus dorsi muscle in two pig breeds
title_full Identification and characterization of circRNAs related to meat quality during embryonic development of the longissimus dorsi muscle in two pig breeds
title_fullStr Identification and characterization of circRNAs related to meat quality during embryonic development of the longissimus dorsi muscle in two pig breeds
title_full_unstemmed Identification and characterization of circRNAs related to meat quality during embryonic development of the longissimus dorsi muscle in two pig breeds
title_short Identification and characterization of circRNAs related to meat quality during embryonic development of the longissimus dorsi muscle in two pig breeds
title_sort identification and characterization of circrnas related to meat quality during embryonic development of the longissimus dorsi muscle in two pig breeds
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705349/
https://www.ncbi.nlm.nih.gov/pubmed/36457752
http://dx.doi.org/10.3389/fgene.2022.1019687
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