Comparing the efficiency of six clearing methods in developing seeds of Arabidopsis thaliana

KEY MESSAGE: ClearSee alpha and FAST9 were optimized for imaging Arabidopsis seeds up to the torpedo stages. The methods preserve the fluorescence of reporter proteins and seed shape, allowing phenotyping embryos in intact seeds. ABSTRACT: Tissue clearing methods eliminate the need for sectioning, t...

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Detalles Bibliográficos
Autores principales: Attuluri, Venkata Pardha Saradhi, Sánchez López, Juan Francisco, Maier, Lukáš, Paruch, Kamil, Robert, Hélène S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705463/
https://www.ncbi.nlm.nih.gov/pubmed/36378346
http://dx.doi.org/10.1007/s00497-022-00453-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705463/
https://www.ncbi.nlm.nih.gov/pubmed/36378346
http://dx.doi.org/10.1007/s00497-022-00453-4

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